{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:46:50Z","timestamp":1788403610210,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T00:00:00Z","timestamp":1730160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:p>The Mpox virus (or Monkeypox virus, MPXV) uses its own encoded proteins to form a replication machine that replicates the viral genome in the host cell cytoplasm, making this machinery a key target for antiviral drug design. The D5 (also known as the OPG117 or E5) protein, a bi\u2010functional helicase\u2013primase enzyme, is crucial in the MPXV replication machinery and genome uncoating process. Recently, cryo\u2010electron microscopy (cryo\u2010EM) structures of D5 in multiple states have been determined. These structures have elucidated the full trajectory of the MPXV D5 helicase\u2013primase as it moves along single\u2010stranded DNA, providing unprecedented advancements in the molecular dynamics and unwinding mechanism. This structural snapshot describes the structural features of the D5 protein and dissects the broader implications of its pivotal role in MPXV replication.<\/jats:p>","DOI":"10.1111\/febs.17292","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T05:30:26Z","timestamp":1730266226000},"page":"510-518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A structural snapshot of the multiple working states of the Mpox virus helicase\u2013primase <scp>D5<\/scp>"],"prefix":"10.1111","volume":"292","author":[{"given":"Yingying","family":"Guo","sequence":"first","affiliation":[{"name":"Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Health Sciences Hainan University  Haikou China"},{"name":"Collaborative Innovation Center of One Health Hainan University  Haikou China"},{"name":"Hainan One Health Key Laboratory Hainan University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7122-6547","authenticated-orcid":false,"given":"Renhong","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Key University Laboratory of Metabolism and Health of Guangdong, School of Medicine, Institute for Biological Electron Microscopy Southern University of Science and Technology  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aah6317"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-5037-5_4"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2004.12.001"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10409238.2021.1954597"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-018-0024-x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-023-01504-1"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.76.052305.115300"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.7.6.13570"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-052610-094414"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.17.3389"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.61.10.3152-3162.1987"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-023-01142-0"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-091919-104752"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M511239200"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02217-06"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00044-16"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14102206"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.9.5353-5361.1995"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-022-00775-4"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2022.102855"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bsheal.2022.11.001"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200404000-00003"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.12.008"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz893"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.09.017"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403454101"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2024.29.33.240815v"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-024-02607-y"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.ade6360"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adg2331"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2023.10.038"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2024.03.004"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01533-12"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709276104"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25843-6"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad677"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011652"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a010199"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.17292","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T04:43:08Z","timestamp":1739248988000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.17292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,29]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1111\/febs.17292"],"URL":"https:\/\/doi.org\/10.1111\/febs.17292","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,29]]},"assertion":[{"value":"2024-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}