{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:30:40Z","timestamp":1792114240056,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,1,19]],"date-time":"2025-01-19T00:00:00Z","timestamp":1737244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81602442"],"award-info":[{"award-number":["81602442"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:p>\n                    Lactate dehydrogenase A (LDHA) is upregulated in multiple cancer types and contributes to the Warburg effect. Several studies have found that many tumor\u2010related genes have subtypes and play important roles in promoting cancer development. Here, we identified a novel LDHA transcript, which produced a new protein 3\u2009kDa larger than LDHA, which we named LDHA\u03b1. We found that multiple cancer cell lines express LDHA\u03b1, and ectopic expression of LDHA\u03b1 led to a higher proliferation and migration rate\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Ectopic expression of LDHA\u03b1 could also promote tumor cell growth\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Conversely, deletion of LDHA\u03b1 by CRISPR\u2010sgRNA significantly inhibited the growth of tumor cells. LDHA\u03b1 was found to be mainly located in the cytoplasm, and overexpression or deletion of LDHA\u03b1 could significantly affect the glucose uptake and lactate production of tumor cells. Further investigation showed that c\u2010MYC and FOXM1 could markedly modulate the expression of both LDHA and LDHA\u03b1, especially c\u2010MYC. We found that a small molecular compound targeting LDHA could also inhibit the enzyme activity of LDHA\u03b1. LDHA\u03b1, LDHA and c\u2010MYC expression was significantly higher in human acute lymphocytic leukemia and colorectal cancer tissue specimens compared to normal controls. In conclusion, our study identified LDHA\u03b1 as a subtype of LDHA and highlighted its critical role in tumor metabolism, providing a potential new therapeutic target for tumor diagnosis and treatment.\n                  <\/jats:p>","DOI":"10.1111\/febs.17374","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T00:37:06Z","timestamp":1737333426000},"page":"2223-2236","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<scp>LDHA\u03b1<\/scp>\n                    , a lactate dehydrogenase A isoform, promotes glycolysis and tumor progression"],"prefix":"10.1111","volume":"292","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8489-6064","authenticated-orcid":false,"given":"Bingqing","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, 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