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As clinical and biotechnological uses of ECM are expanding, it is essential to assess the environmental impact associated with its production. Due to high levels of customization, various laboratories employ distinct methods; therefore, this study evaluates three common protocols. Life cycle assessment (LCA) methodology has been developed to evaluate the environmental impacts of products produced through diverse processes. Despite its widespread application in the pharmaceutical industry, LCA has seldom been utilized to estimate the environmental effects of laboratory protocols. This Viewpoint applies LCA to assess the functionality and environmental impacts of ECM produced via P1, P2, and P3. The results of this assessment indicate that the protocol with the highest impact generates approximately 43 times more CO<jats:sub>2<\/jats:sub>\u2010equivalent emissions (CO<jats:sub>2<\/jats:sub> eq) than that with the lowest impact, while the ECM produced using the least impactful protocol demonstrates the highest biocompatibility. Additional environmental indicators such as eutrophication, photochemical oxidation, and acidification also vary among the tested protocols. This work underscores the need to factor environmental impact in the development of novel biomedical materials.<\/jats:p>","DOI":"10.1111\/febs.17385","type":"journal-article","created":{"date-parts":[[2025,1,5]],"date-time":"2025-01-05T11:58:26Z","timestamp":1736078306000},"page":"2208-2218","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The environmental impact of extracellular matrix preparation"],"prefix":"10.1111","volume":"292","author":[{"given":"Ying","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Biomaterials The First Affiliated Hospital of Ningbo university  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zihao","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine The First Affiliated Hospital of Ningbo university  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