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Recently, GlcNAc has been shown to promote plant growth by shaping the bacterial soil community. However, the role of GlcNAc sensing in filamentous soil fungi has not been investigated. By using\n                    <jats:italic>Trichoderma reesei<\/jats:italic>\n                    as a model organism, we show here that GlcNAc impacts the expression of around 2100 genes. Carbohydrate metabolism, amino acid metabolism, and secondary metabolism were the three most strongly affected classes of eukaryotic orthologous groups (KOG classes). Two key regulators of GlcNAc catabolism, the NDT80 domain\u2010containing transcriptional regulator RON1, and a GlcNAc sensor, NGS1, are needed for differential regulation of two\u2010thirds of these genes.\n                    <jats:italic>In silico<\/jats:italic>\n                    structural modeling of NGS1 identified a domain with homology to the GCN5\u2010related histone acetyltransferase from\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , which serves as a GlcNAc catabolism regulator and GlcNAc sensor. Finally, we characterized the third regulator of GlcNAc sensing in\n                    <jats:italic>T. reesei<\/jats:italic>\n                    , which is the highly specific GlcNAc transporter\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetylglucosamine transporter (NGT1). Using a deletion mutant of\n                    <jats:italic>ngt1<\/jats:italic>\n                    , we demonstrate that GlcNAc has to enter the cell to activate the GlcNAc catabolic gene expression. Interestingly, in contrast to dimorphic yeasts, the pathways for defense and pathogenicity seem to be induced in\n                    <jats:italic>T. reesei<\/jats:italic>\n                    by external GlcNAc. Given the ancestral role of\n                    <jats:italic>Trichoderma<\/jats:italic>\n                    spp. in the fungal kingdom and the highly conserved GlcNAc catabolism cluster that includes their regulators in many species of fungi, we propose a regulatory network for GlcNAc sensing in soil fungi.\n                  <\/jats:p>","DOI":"10.1111\/febs.70015","type":"journal-article","created":{"date-parts":[[2025,2,15]],"date-time":"2025-02-15T12:20:31Z","timestamp":1739622031000},"page":"3072-3090","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>N<\/i>\n                    \u2010acetylglucosamine sensing in the filamentous soil fungus\n                    <i>Trichoderma reesei<\/i>"],"prefix":"10.1111","volume":"292","author":[{"given":"Sadia Fida","family":"Ullah","sequence":"first","affiliation":[{"name":"Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology AlbaNova 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