{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:00:52Z","timestamp":1790694052557,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T00:00:00Z","timestamp":1747612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004770","name":"Universit\u00e0 degli Studi di Parma","doi-asserted-by":"publisher","award":["Bando di Ateneo 2021 per la ricerca"],"award-info":[{"award-number":["Bando di Ateneo 2021 per la ricerca"]}],"id":[{"id":"10.13039\/501100004770","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:p>\n                    Phosphonates are organic molecules containing a direct carbon\u2013phosphorus (C\u2013P) bond. They are chemically sturdy compounds that can, however, be degraded by environmental microorganisms. In the frame of bacterial phosphonate catabolism, we recently reported the discovery of (\n                    <jats:italic>R<\/jats:italic>\n                    )\u20101\u2010hydroxy\u20102\u2010aminoethylphosphonate ammonia\u2010lyase (PbfA), a lyase acting on the natural compound (\n                    <jats:italic>R<\/jats:italic>\n                    )\u20102\u2010amino\u20101\u2010hydroxyethylphosphonate (\n                    <jats:italic>R<\/jats:italic>\n                    \u2010HAEP). PbfA converts\n                    <jats:italic>R<\/jats:italic>\n                    \u2010HAEP into phosphonoacetaldehyde (PAA), which can be subsequently processed and cleaved by further enzymes. However, PbfA is not active toward\n                    <jats:italic>S<\/jats:italic>\n                    \u2010HAEP (the enantiomer of\n                    <jats:italic>R<\/jats:italic>\n                    \u2010HAEP), whose metabolic fate remained unknown. We now describe the identification of a racemase, discovered through genomic context analysis, which converts\n                    <jats:italic>S<\/jats:italic>\n                    \u2010HAEP into\n                    <jats:italic>R<\/jats:italic>\n                    \u2010HAEP, thereby enabling degradation of\n                    <jats:italic>S<\/jats:italic>\n                    \u2010HAEP. We propose for this enzyme the official name 2\u2010amino\u20101\u2010hydroxyethylphosphonate racemase (shorthand PbfF). To our knowledge, PbfF is the first NAD\u2010dependent racemase ever described and is structurally unrelated to other known NAD\u2010dependent isomerases. The enzyme uses NAD\n                    <jats:sup>+<\/jats:sup>\n                    as a cofactor, is inhibited by NADH, and shows catalytic parameters comparable to those of other racemases acting on similar substrates. The presence of a pathway for the breakdown of\n                    <jats:italic>S<\/jats:italic>\n                    \u2010HAEP in numerous bacteria suggests that this compound may be more common in the environment than currently appreciated. Notably, the route for\n                    <jats:italic>S<\/jats:italic>\n                    \u2010HAEP degradation appears to have developed through a mechanism of retrograde metabolic evolution.\n                  <\/jats:p>","DOI":"10.1111\/febs.70130","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T05:04:54Z","timestamp":1747631094000},"page":"4272-4288","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genomic context analysis enables the discovery of an unusual\n                    <scp>NAD<\/scp>\n                    \u2010dependent racemase in phosphonate catabolism"],"prefix":"10.1111","volume":"292","author":[{"given":"Francesca","family":"Ruffolo","sequence":"first","affiliation":[{"name":"Department of Chemistry, Life Sciences and Environmental Sustainability University of Parma  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