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PpiC is a membrane\u2010bound lipoprotein of\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecium<\/jats:italic>\n                    endowed with both a peptidyl\u2010prolyl isomerase and a foldase activity, and plays a key role in assisting the folding of many secreted enterococcal proteins. It is located at the membrane\u2013wall interface, therefore easily accessible to inhibitors and to the immune system and an ideal target for drug and vaccine development. Despite their potential, enterococcal peptidyl\u2010prolyl isomerases have been understudied. We previously identified PpiC as an important cross\u2010protective vaccine antigen. To gain a better understanding of the PpiC biological role in\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecium<\/jats:italic>\n                    survival, we determined the crystal structure of PpiC and investigated its biophysical properties. Consistent with PpiC's folding activity, the biological assembly of PpiC is a bowl\u2010shaped structure containing two parvulin\u2010type peptidyl\u2010prolyl cis\/trans isomerase domains. We also dissected the role of\n                    <jats:italic>N<\/jats:italic>\n                    \u2010 and\n                    <jats:italic>C<\/jats:italic>\n                    \u2010terminal regions of the molecule in its dimerisation, an event which is predicted to play an important role in the folding of client proteins. Our data point to a functional cross\u2010talk between the foldase and peptidyl\u2010prolyl isomerase activities of PpiC, through the protein\u2010swapping involved in dimerisation. Also, our work provides key structural data for the design of antimicrobials and cross\u2010protective vaccine antigens against nosocomial infections.\n                  <\/jats:p>","DOI":"10.1111\/febs.70160","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T02:19:01Z","timestamp":1751336341000},"page":"5475-5490","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Crystal structure and biophysical characterisation of the enterococcal foldase\n                    <scp>PpiC<\/scp>\n                    , a cross\u2010opsonic antigen against gram\u2010positive nosocomial pathogens"],"prefix":"10.1111","volume":"292","author":[{"given":"Valeria","family":"Napolitano","sequence":"first","affiliation":[{"name":"Institute of Biostructures and Bioimaging, Italian Research Council (CNR)  Naples 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