{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:36:42Z","timestamp":1788345402553,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["310030_219605"],"award-info":[{"award-number":["310030_219605"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["320030_219451"],"award-info":[{"award-number":["320030_219451"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013362","name":"Swiss Cancer Research Foundation","doi-asserted-by":"publisher","award":["UniBE KFS\u20105936\u201008\u20102023\u2010R"],"award-info":[{"award-number":["UniBE KFS\u20105936\u201008\u20102023\u2010R"]}],"id":[{"id":"10.13039\/501100013362","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000850","name":"National Heart and Lung Institute","doi-asserted-by":"publisher","award":["PO1HL171803"],"award-info":[{"award-number":["PO1HL171803"]}],"id":[{"id":"10.13039\/501100000850","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM140210"],"award-info":[{"award-number":["R01GM140210"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R24GM137763"],"award-info":[{"award-number":["R24GM137763"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01CA199882"],"award-info":[{"award-number":["U01CA199882"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:p>\n                    The H antigen (O blood group), the precursor to A and B blood groups, is expressed on human erythrocytes and other cells (e.g., endothelial cells). Remarkably, the specific expression of the H antigens is uncertain due to the lack of specific reagents for its detection. Here, we describe two monoclonal antibodies (mAbs), Tn4\u201031L and OmcFL3\u201002, generated by immunizing sea lampreys with human cells. Both mAbs are highly specific in comparison with the lectin\n                    <jats:italic>Ulex europaeus<\/jats:italic>\n                    agglutinin\u2010I (UEA\u2010I). We identified expression of H antigens in mammalian glycoproteins, human cells, and normal and malignant human tissues, discovering that different types of human carcinomas exhibit unexpected H antigen expression. Interestingly, H antigen detection in many cases was distinctly elevated by enzymatic desialylation, as well as being elevated in cells engineered to lack sialic acid. These highly specific mAbs will be valuable reagents in blood testing and for exploring the expression and function of H antigens.\n                  <\/jats:p>","DOI":"10.1111\/febs.70172","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T02:18:48Z","timestamp":1751336328000},"page":"5697-5722","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Blood group O expression in normal tissues and tumors"],"prefix":"10.1111","volume":"292","author":[{"given":"Ea Kristine Clarisse","family":"Tulin","sequence":"first","affiliation":[{"name":"Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"Muerner","sequence":"additional","affiliation":[{"name":"Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School  Boston MA USA"},{"name":"Institute of Pharmacology, University of Bern, Inselspital, INO\u2010F  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanya R.","family":"McKitrick","sequence":"additional","affiliation":[{"name":"Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A.","family":"Tilton","sequence":"additional","affiliation":[{"name":"Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren E.","family":"Pepi","sequence":"additional","affiliation":[{"name":"Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianwei","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxence","family":"Noel","sequence":"additional","affiliation":[{"name":"Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon Y.","family":"Zheng","sequence":"additional","affiliation":[{"name":"Joint Program in Transfusion Medicine Brigham and Women's Hospital, Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean R.","family":"Stowell","sequence":"additional","affiliation":[{"name":"Joint Program in Transfusion Medicine Brigham and Women's Hospital, Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"von Gunten","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology, University of Bern, Inselspital, INO\u2010F  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Heimburg\u2010Molinaro","sequence":"additional","affiliation":[{"name":"Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8918-5034","authenticated-orcid":false,"given":"Richard D.","family":"Cummings","sequence":"additional","affiliation":[{"name":"Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,30]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"1132","article-title":"\u00fcber Agglutinationserscheinungen normalen menschlichen Blutes","volume":"14","author":"Landsteiner K","year":"1901","journal-title":"Wien Klin Wochenschr"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/169825a0"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011014307683"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0950-3536(05)80155-6"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1989.tb03040.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-056"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.105798"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12820"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12786"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12787"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15020471"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.940196"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14681"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-94"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1306070110"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42669-X"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/83.3.308"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215549804600109"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00731501"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000204150"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-6247.172180"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-0819-2"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1944-5_5"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2017.09.003"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02199-7"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M117.067983"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1993.tb18370.x"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900850"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.691217"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baw100"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/glycobiology.4e.14"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)32147-1"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19910201)67:3<613::AID-CNCR2820670316>3.0.CO;2-I"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19930901)72:5<1581::AID-CNCR2820720515>3.0.CO;2-B"},{"key":"e_1_2_10_36_1","first-page":"2183","article-title":"Highly tumor\u2010reactive, internalizing, mouse monoclonal antibodies to Le(y)\u2010related cell surface antigens","volume":"50","author":"Hellstrom I","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.05731.x"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(03)00022-1"},{"key":"e_1_2_10_39_1","first-page":"903","article-title":"A \u201cnew\u201d blood group character related to the ABO system","volume":"1","author":"Bhende YM","year":"1952","journal-title":"Lancet"},{"key":"e_1_2_10_40_1","volume-title":"The Blood Group Antigen Factsbook","author":"Reid ME","year":"2012"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.799519"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/bjs\/znac293"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-47131-9"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10031"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00688133"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00227a023"},{"key":"e_1_2_10_47_1","first-page":"1295","article-title":"Generation of monoclonal antibodies reacting with normal and cancer cells of human breast","volume":"43","author":"Menard S","year":"1983","journal-title":"Cancer Res"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68541-8"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11091241"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-01501-1"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-044453077-6\/50017-X"},{"key":"e_1_2_10_52_1","first-page":"645","volume-title":"Essentials of Glycobiology","author":"Cummings RD","year":"2022"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.9721"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.1c00822"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.4.2089"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwx101"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3047"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.4c02124"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1012"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.70172","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T03:35:39Z","timestamp":1762486539000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.70172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,30]]},"references-count":58,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/febs.70172"],"URL":"https:\/\/doi.org\/10.1111\/febs.70172","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,30]]},"assertion":[{"value":"2025-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}