{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T23:44:35Z","timestamp":1785714275632,"version":"3.56.0"},"reference-count":51,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["CRG\/2020\/001649"],"award-info":[{"award-number":["CRG\/2020\/001649"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:p>\n                    The ubiquitous\n                    <jats:italic>ARV1<\/jats:italic>\n                    gene shows significant functional conservation across eukaryotes.\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    Arv1 is implicated in several cellular processes, including lipid\/sterol homeostasis, morphogenesis, and drug resistance. Human and fungal\n                    <jats:italic>ARV1<\/jats:italic>\n                    functionally complement\n                    <jats:italic>S. cerevisiae ARV1<\/jats:italic>\n                    , and\n                    <jats:italic>arv1<\/jats:italic>\n                    \u0394 is rescued by the overexpression of some subunits of the GPI\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetylglucosaminyltransferase (GPI\u2010GnT), which catalyzes the first GPI biosynthetic step. Human and\n                    <jats:italic>Trypanosoma brucei<\/jats:italic>\n                    Arv1 homologs co\u2010immunoprecipitate with different GPI\u2010GnT subunits. Based on these previous reports, we hypothesized a cross talk between\n                    <jats:italic>Candida albicans ARV1<\/jats:italic>\n                    and the first step of GPI biosynthesis. Using super\u2010resolution radial fluctuation (SRRF) analysis of co\u2010localization data, co\u2010immunoprecipitation assays, and acceptor\u2010photobleaching FRET studies, we show that CaArv1 physically interacts with the GPI\u2010GnT. It also regulates the expression of the GPI\u2010GnT subunits via the epigenetic modulator, Rtt109. Overexpressing\n                    <jats:italic>GPI19<\/jats:italic>\n                    (which encodes a GPI\u2010GnT subunit whose expression is repressed in\n                    <jats:italic>Caarv1<\/jats:italic>\n                    \u0394\/\u0394) rescues its cell wall phenotype, sensitivity to azoles, and GPI\u2010GnT activity without reversing the filamentation defect. A similar rescue is observed on downregulating\n                    <jats:italic>GPI2<\/jats:italic>\n                    (encoding another GPI\u2010GnT subunit, whose expression is upregulated in\n                    <jats:italic>Caarv1<\/jats:italic>\n                    \u0394\/\u0394). Thus, transcriptional control rather than physical interaction appears to be the primary mechanism by which CaArv1 controls GPI\u2010GnT. Overexpressing\n                    <jats:italic>RAS1<\/jats:italic>\n                    restores all phenotypes, including filamentation, without restoring GPI\u2010GnT activity. The filamentation defect of\n                    <jats:italic>Caarv1<\/jats:italic>\n                    \u0394\/\u0394 is independent of the GPI\u2010GnT. CaArv1 transcriptionally regulates hyphae\u2010specific transcription factors downstream of cAMP\u2010PKA signaling (Efg1, Flo8) and repressors (Tup1, Nrg1) to modulate filamentation. The cross talk between CaArv1 and GPI\u2010GnT has important implications for the virulence of\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>albicans<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/febs.70217","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T11:32:41Z","timestamp":1755084761000},"page":"6710-6734","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Arv1 interacts with and regulates the first step of\n                    <scp>GPI<\/scp>\n                    biosynthesis in\n                    <i>Candida albicans<\/i>"],"prefix":"10.1111","volume":"292","author":[{"given":"Monika","family":"Bharati","sequence":"first","affiliation":[{"name":"School of Life Sciences Jawaharlal Nehru University  New Delhi 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