{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T20:27:59Z","timestamp":1773865679484,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["159923"],"award-info":[{"award-number":["159923"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["192246"],"award-info":[{"award-number":["192246"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000156","name":"Fonds de Recherche du Qu\u00e9bec - Sant\u00e9","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:p>\n                    <jats:italic>Leishmania donovani<\/jats:italic>\n                    is a causative agent of the neglected tropical disease known as visceral leishmaniasis or Kala Azar. This disease is lethal when untreated, with more than 90\u2009000 cases annually. Little is known about magnesium regulation in these parasites despite magnesium being the second most abundant intracellular cation and universally required for normal cell function.\n                    <jats:italic>L.\u2009donovani<\/jats:italic>\n                    contains two protein tyrosine phosphatase (PTP) proteins (PTP1 and PTP2) and a DUF21 protein (domain of unknown function 21), which are respectively homologous to mammalian PRL [phosphatases of regenerating liver; also known as protein tyrosine phosphatase type IVA (PTP4A)] and mammalian transmembrane protein CNNM (cyclin M family). In mammalian cells, the PRL and CNNM multiprotein complex has been shown to sense and modulate intracellular magnesium levels. Herein, we revealed that\n                    <jats:italic>L.\u2009donovani<\/jats:italic>\n                    PTP1 and DUF21 can also form a specific protein complex. Using CRISPR\u2010Cas9 gene editing, four\n                    <jats:italic>L.\u2009donovani<\/jats:italic>\n                    knockouts, Ld\u0394PTP1, Ld\u0394PTP2, a double knockout termed Ld\u0394PTP1\/2, and Ld\u0394DUF21 have been generated. Magnesium\u2010dependent growth curves demonstrated that the Ld\u0394PTP1\/2 mutant could not survive in low magnesium and had a reduced level of survival in infected macrophages. In contrast, Ld\u0394DUF21 is sensitive to high levels of magnesium and has an increased level of intracellular magnesium and an increased survival in macrophages compared to wild\u2010type\n                    <jats:italic>L.\u2009donovani.<\/jats:italic>\n                    Taken together, these observations provide evidence that, similar to the PRL and CNNM proteins in mammalian cells, PTP and DUF21 homologs in\n                    <jats:italic>L.\u2009donovani<\/jats:italic>\n                    have the ability to complex and respond to environmental changes in magnesium.\n                  <\/jats:p>","DOI":"10.1111\/febs.70287","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T20:09:05Z","timestamp":1760731745000},"page":"1754-1770","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Leishmania donovani<\/i>\n                    's protein tyrosine phosphatases interact with\n                    <scp>DUF<\/scp>\n                    21 and respond to environmental magnesium"],"prefix":"10.1111","volume":"293","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9379-9509","authenticated-orcid":false,"given":"Kayla","family":"Paulini","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology McGill University  Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8438-6614","authenticated-orcid":false,"given":"Hira","family":"Khursheed","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Goodman Cancer Institute McGill University  Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1996-876X","authenticated-orcid":false,"given":"Wen Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology McGill University  Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5295-7418","authenticated-orcid":false,"given":"Isabelle","family":"Aubry","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Goodman Cancer Institute McGill University  Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8971-5525","authenticated-orcid":false,"given":"Greg","family":"Matlashewski","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology McGill University  Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0281-541X","authenticated-orcid":false,"given":"Michel L.","family":"Tremblay","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Goodman Cancer Institute McGill University  Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5550-712X","authenticated-orcid":false,"given":"Patrick","family":"Lypaczewski","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology McGill University  Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31204-2"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2023.01.057"},{"key":"e_1_2_10_4_1","unstructured":"M\u00e9decins Sans Fronti\u00e8res Access Campaign(2021)Liposomal amphotericin B: solving the access puzzle."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(24)00225-0"},{"key":"e_1_2_10_6_1","volume-title":"Ending the Neglect to Attain the Sustainable Development Goals: A Road Map for Neglected Tropical Diseases 2021\u20132030","author":"World Health Organization","year":"2020"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndtplus\/sfr163"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2009.0179"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-004-0646-4"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01930-5"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.11.010"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00084-17"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14503"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.33"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2221083120"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2021.09.004"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2021.128167"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-17-1510"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.759944"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-00147-2"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201643393"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.705863"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0294-2_14"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00156-22"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn1069"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00599-17"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2016.04.018"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010848"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb02197.x"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E04-12-1096"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00052-22"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-55538-7"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.05.020"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.11.006"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102370"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-019-0682-0"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-022-00618-w"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.5539\/ijc.v6n1p35"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)01043-2"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae241"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed7110384"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009079"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07230.x"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1727-y"},{"key":"e_1_2_10_45_1","article-title":"Evaluation of the accuracy of bacterial genome reconstruction with Oxford Nanopore R10.4.1 long\u2010read\u2010only sequencing","volume":"10","author":"Sanderson ND","year":"2024","journal-title":"Microb Genom"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-34812-x"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-00178-w"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq033"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003983"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.70287","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/febs.70287","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.70287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T12:59:30Z","timestamp":1773838770000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.70287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,17]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/febs.70287"],"URL":"https:\/\/doi.org\/10.1111\/febs.70287","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,17]]},"assertion":[{"value":"2025-04-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}