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In this investigation, we performed a comprehensive analysis of the role of an intron from two yeast genes,\n                    <jats:italic>ASC1<\/jats:italic>\n                    and\n                    <jats:italic>APE2<\/jats:italic>\n                    , on cellular fitness and at different steps of gene expression. Mutants containing intronless versions of either gene grew 30\u201360% slower than their intron\u2010containing counterparts, and their growth was compromised on ethanol and glycerol medium. Furthermore, there was an enhanced R\u2010loop signal in the coding region of both genes in the absence of the intron. Steady\u2010state RNA levels of\n                    <jats:italic>ASC1<\/jats:italic>\n                    and\n                    <jats:italic>APE2<\/jats:italic>\n                    decreased by about 30\u2010fold and 5\u2010fold, respectively, in the absence of the intron. Nascent transcription analysis revealed a drop in transcription of both\n                    <jats:italic>ASC1<\/jats:italic>\n                    and\n                    <jats:italic>APE2<\/jats:italic>\n                    by 4\u201310 fold and 2\u20135 fold, respectively, in the intronless state. The half\u2010life of mRNA of both genes registered a 2\u2010 to 3\u2010fold decline in the absence of an intron. A fluorescence\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization approach detected an increase in nuclear retention of mRNA in the absence of the intron for both genes. Measurement of protein level by western blot found no detectable signal for either protein in the absence of an intron. These results suggest that the introns of both genes affect the expression of their genes at the level of transcription, mRNA stability, and nucleocytoplasmic transport of mRNA. Furthermore, both\n                    <jats:italic>ASC1<\/jats:italic>\n                    and\n                    <jats:italic>APE2<\/jats:italic>\n                    introns affect the fitness of cells in terms of growth rate and the ability to grow on different carbon sources.\n                  <\/jats:p>","DOI":"10.1111\/febs.70294","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T04:15:03Z","timestamp":1761279303000},"page":"1238-1251","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive analysis of the role of\n                    <i>ASC1<\/i>\n                    and\n                    <i>APE2<\/i>\n                    introns on cellular fitness, transcription, and post\u2010transcriptional dynamics"],"prefix":"10.1111","volume":"293","author":[{"given":"Emma","family":"Fidler","sequence":"first","affiliation":[{"name":"Department of Biological Science Wayne State University  Detroit MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zulifa","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biological Science Wayne State University  Detroit MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahlam","family":"Awada","sequence":"additional","affiliation":[{"name":"Department of Biological Science Wayne State University  Detroit MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Dwyer","sequence":"additional","affiliation":[{"name":"Department of Biological Science Wayne State University  Detroit MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susheel","family":"Pangeni","sequence":"additional","affiliation":[{"name":"Department of Physics Wayne State University  Detroit MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Physics Wayne State University  Detroit MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2806-421X","authenticated-orcid":false,"given":"Athar","family":"Ansari","sequence":"additional","affiliation":[{"name":"Department of Biological Science Wayne State University  Detroit MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2012.00055"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08909"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.07.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0828-1"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0859-7"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(03)00052-5"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-76776-3_15"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2015.04.017"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.01977"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2017.06.016"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2019.194439"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-020-0258-4"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3662"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2012.08.004"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2018.00672"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1997.11030455.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.5250403"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.5260303"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M212746200"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.12.184"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-017-1791-x"},{"key":"e_1_2_10_23_1","first-page":"4404","article-title":"Role of RNA splicing in mediating lineage\u2010specific expression of the von Willebrand factor gene in the endothelium. 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