{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T21:30:32Z","timestamp":1793223032803,"version":"4.5.0"},"reference-count":69,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170698"],"award-info":[{"award-number":["82170698"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82500834"],"award-info":[{"award-number":["82500834"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:p>\n                    Acute kidney injury (AKI) is a common and often fatal condition characterized by tubular epithelial cell necrosis and immune cell infiltration. The macrophage (M\u0424) plays multiple roles in kidney injury and repair after AKI. However, the classification and function of M\u0424 subsets involved in AKI remain poorly understood. In this study, kidney bulk\u2010sequencing showed the injury pattern (day 0 to day 3) to repair pattern (day 4 to day 7) after cisplatin\u2010induced AKI (Cis\u2010AKI). Single\u2010cell RNA sequencing of Cis\u2010AKI mouse kidneys dissected the transcriptome heterogeneity of renal M\u0424s, and depletion and adoptive transfer experiments were used to explore the functional distinction of M\u0424 subsets. The single\u2010cell atlas identified four M\u0424 subsets with distinct transcriptomic profiles (Mo\u2010M\u0424, Mki67\n                    <jats:sup>+<\/jats:sup>\n                    M\u0424, Cd74\n                    <jats:sup>+<\/jats:sup>\n                    M\u0424, Cx3cr1\n                    <jats:sup>+<\/jats:sup>\n                    M\u0424). The dynamic proportion change of M\u0424 subsets from progression to regression analyses revealed that Mo\u2010M\u0424, defined as recruit\u2010M\u0424 in this study, was primarily distributed in the progression stage, whereas the other M\u0424 subsets, defined as resident\u2010M\u0424, were dominant in the non\u2010pathological condition and regression stage. By depletion and adoptive transfer experiments, our results confirmed that the Cx3cr1\n                    <jats:sup>+<\/jats:sup>\n                    M\u0424 subset plays a crucial role in the protection against Cis\u2010AKI. Further cell coculture experiments revealed that the tyrosine\u2010protein kinase receptor UFO (AXL)\u2013growth arrest\u2010specific protein 6 (GAS6) ligand\u2013receptor pair is involved in regulating the efferocytosis of apoptotic renal tubular epithelial cells by Cx3cr1\n                    <jats:sup>+<\/jats:sup>\n                    M\u0424s, representing one of the molecular mechanisms in promoting kidney repair. In conclusion, our study dissected the heterogeneity of renal macrophage subsets during Cis\u2010AKI, and the discovery of the protective Cx3cr1\n                    <jats:sup>+<\/jats:sup>\n                    M\u0424 subset may provide new therapeutic targets for AKI intervention.\n                  <\/jats:p>","DOI":"10.1111\/febs.70302","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:19:13Z","timestamp":1761664753000},"page":"963-985","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Single\u2010cell\n                    <scp>RNA<\/scp>\n                    sequencing reveals the protective role of renal Cx3cr1\n                    <sup>+<\/sup>\n                    macrophages in cisplatin\u2010induced acute kidney injury"],"prefix":"10.1111","volume":"293","author":[{"given":"Bingquan","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Nephrology, Sir Run Run Hospital Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4024-6684","authenticated-orcid":false,"given":"Mengqing","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Nanjing First Hospital Nanjing Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijuan","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Sir Run Run Hospital Nanjing Medical University  China"},{"name":"Department of Nephrology First People's Hospital of Jiujiang City  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Nanjing First Hospital Nanjing Medical University  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