{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:30:53Z","timestamp":1781109053307,"version":"3.54.1"},"reference-count":18,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:p>\n                    Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly infectious RNA virus that severely affects swine herds, causing respiratory and reproductive disorders with major global economic impact. Current first\u2010generation vaccines based on inactivated or attenuated viruses offer limited protection and may pose safety risks. Given the virus's high mutation rate and genetic variability, there is a growing need for safer and more effective vaccines. Plant molecular farming represents a promising alternative production system for second\u2010generation subunit vaccines, offering advantages in terms of cost, biosafety, and scalability. Recent studies have explored the coupling of PRRSV antigens to virus\u2010like (VLP) or nonviral self\u2010assembling protein nanoparticles (PNPs) to enhance immunogenicity. VanderBurgt\n                    <jats:italic>et\u00a0al.<\/jats:italic>\n                    now provide a successful example of the production of both VLP and PNP vaccines against PRRSV in plants, with preliminary\n                    <jats:italic>in vivo<\/jats:italic>\n                    results showing encouraging immune responses.\n                  <\/jats:p>","DOI":"10.1111\/febs.70373","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T18:04:46Z","timestamp":1765821886000},"page":"1886-1890","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Current advances in recombinant porcine reproductive and respiratory syndrome virus vaccines in plants"],"prefix":"10.1111","volume":"293","author":[{"given":"Marco","family":"Costantini","sequence":"first","affiliation":[{"name":"Division of Biotechnology ENEA Research Center Casaccia  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9183-0728","authenticated-orcid":false,"given":"Marcello","family":"Donini","sequence":"additional","affiliation":[{"name":"Division of Biotechnology ENEA Research Center Casaccia  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines13030260"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines12080942"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci10080491"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-021-00330-7"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines13020191"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/v16060936"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2009.12.003"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12015"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.01.059"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-011-1523-8"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-017-2836-z"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091386"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2015.07.012"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1044675"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.70288"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c12212"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/v16060991"},{"key":"e_1_2_10_19_1","first-page":"1170","article-title":"Expression of the Multimeric and highly immunogenic Brucella spp. Lumazine synthase fused to bovine rotavirus VP8d as a scaffold for antigen production in tobacco chloroplasts","volume":"23","author":"Alfano EF","year":"2015","journal-title":"Front Plant Sci"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.70373","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/febs.70373","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.70373","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T11:05:59Z","timestamp":1775127959000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.70373"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,15]]},"references-count":18,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1111\/febs.70373"],"URL":"https:\/\/doi.org\/10.1111\/febs.70373","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,15]]},"assertion":[{"value":"2025-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}