{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T08:13:35Z","timestamp":1779264815465,"version":"3.51.4"},"reference-count":61,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009774","name":"College of Veterinary Medicine, University of Tennessee","doi-asserted-by":"publisher","award":["Start\u2010up funds"],"award-info":[{"award-number":["Start\u2010up funds"]}],"id":[{"id":"10.13039\/100009774","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:p>\n                    <jats:italic>Rickettsia parkeri<\/jats:italic>\n                    rickettsiosis is an emerging tick\u2010borne disease in humans. The bacterium\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    is primarily transmitted to humans by the Gulf Coast tick,\n                    <jats:italic>Amblyomma maculatum<\/jats:italic>\n                    . Upon transmission to the host,\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    infects and multiplies in host endothelial cells. In this study, we provide evidence that\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    modulates the host nicotinamide adenine dinucleotide (NAD+) pathway for its survival in endothelial cells. Analysis performed with arrays containing antibodies against proteins involved in different metabolic pathways revealed increased levels of BST1 (an NAD+\u2010dependent molecule) in\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    \u2010infected endothelial cells compared to the levels noted in uninfected controls. Quantitative PCR analysis further validated antibody array results. We noted modulation of several NAD+ pathway genes and increased reactive oxygen species (ROS) production upon\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    infection in endothelial cells. Treatment of endothelial cells with NAD+ before\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    infection but not postinfection significantly increased ROS levels that affected the bacterial burden. In contrast, we noted increased\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    loads and reduced ROS levels in endothelial cells when bacteria were pre\u2010incubated with NAD+ before infection. Furthermore, siRNA\u2010mediated silencing of\n                    <jats:italic>bst1<\/jats:italic>\n                    expression increased bacterial loads, reduced ROS levels, and affected the expression of NAD+ metabolic pathway genes. Collectively, these results not only elucidate the importance of the host NAD+ metabolic pathway in limiting\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    infection by ROS production but also suggest its therapeutic role in preventing this and perhaps other rickettsial infections of medical importance.\n                  <\/jats:p>","DOI":"10.1111\/febs.70403","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T09:11:22Z","timestamp":1769591482000},"page":"3026-3041","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Rickettsia parkeri<\/i>\n                    subverts nicotinamide adenine dinucleotide (\n                    <scp>NAD<\/scp>\n                    +) metabolic pathway for its survival in endothelial cells"],"prefix":"10.1111","volume":"293","author":[{"given":"Lichao","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine University of Tennessee  Knoxville USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy W.","family":"Turck","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine University of Tennessee  Knoxville USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahid","family":"Karim","sequence":"additional","affiliation":[{"name":"School of Biological, Environmental, and Earth Sciences The University of Southern Mississippi  Hattiesburg USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher D.","family":"Paddock","sequence":"additional","affiliation":[{"name":"Rickettsial Zoonoses Branch Centers for Disease Control and Prevention  Atlanta 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