{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T14:27:41Z","timestamp":1782311261538,"version":"3.54.5"},"reference-count":62,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002426","name":"Fondazione Telethon","doi-asserted-by":"publisher","award":["GMR22T1041"],"award-info":[{"award-number":["GMR22T1041"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009882","name":"Regione Lombardia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009882","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004631","name":"Fondazione Antonio Carlo Monzino","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004631","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:p>\n                    Paired mesoderm homeobox protein 2B (PHOX2B) is a transcription factor essential for autonomic nervous system development. Heterozygous mutations in the\n                    <jats:italic>PHOX2B<\/jats:italic>\n                    gene are associated with neurodevelopmental disorders, including congenital central hypoventilation syndrome and Hirschsprung's disease. Additionally,\n                    <jats:italic>PHOX2B<\/jats:italic>\n                    plays a role in the genetic landscape of neuroblastoma, with mutations detected in both familial and sporadic forms of this rare cancer. Notably,\n                    <jats:italic>PHOX2B<\/jats:italic>\n                    is highly expressed in most neuroblastoma cells. Despite its significance, little is known about the regulation of\n                    <jats:italic>PHOX2B<\/jats:italic>\n                    gene expression, and limited attention has been given to the genomic features of the antisense strand at the\n                    <jats:italic>PHOX2B<\/jats:italic>\n                    locus, although the presence of an antisense transcript is suggested by bioinformatics analyses. In this study, we characterize the recently annotated human antisense transcript\n                    <jats:italic>PHOX2B\u2010AS1<\/jats:italic>\n                    and the previously unidentified mouse antisense\n                    <jats:italic>Phox2b<\/jats:italic>\n                    transcript. Our findings reveal that PHOX2B positively regulates\n                    <jats:italic>PHOX2B\u2010AS1<\/jats:italic>\n                    expression and that inhibiting the antisense transcript reduces PHOX2B protein levels. Together, these results provide strong evidence for the existence of a gene antisense to\n                    <jats:italic>PHOX2B<\/jats:italic>\n                    and highlight a strict correlation and reciprocal regulation between\n                    <jats:italic>PHOX2B<\/jats:italic>\n                    and\n                    <jats:italic>PHOX2B\u2010AS1<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/febs.70410","type":"journal-article","created":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T12:35:55Z","timestamp":1769258155000},"page":"3502-3527","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Regulation of\n                    <i>PHOX2B<\/i>\n                    gene expression by the long non\u2010coding natural antisense\n                    <scp>RNA<\/scp>\n                    <i>PHOX2B\u2010AS1<\/i>"],"prefix":"10.1111","volume":"293","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5971-1515","authenticated-orcid":false,"given":"Simona","family":"Di Lascio","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology and Translational Medicine Universit\u00e0 degli Studi di Milano  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Lucia","family":"Cuadros Gamboa","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology and Translational Medicine Universit\u00e0 degli Studi di Milano  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Bertocchi","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology and Translational Medicine Universit\u00e0 degli Studi di Milano  Milan 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