{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:11:24Z","timestamp":1791562284004,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075\u201015\u20102024\u2010530"],"award-info":[{"award-number":["075\u201015\u20102024\u2010530"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:p>\n                    Cerebral ischaemia is an acute state characterised by a severe decrease in the supply of oxygen (O\n                    <jats:sub>2<\/jats:sub>\n                    ) to the brain, resulting in the death of neurons and glial cells. Despite multiple studies investigating the processes that lead to cell damage under ischaemic stroke, there is still a lack of information about the changes in blood properties under various phases of transient ischaemia with restoration of blood flow or under prolonged ischaemia. Blood, as a source of O\n                    <jats:sub>2<\/jats:sub>\n                    , might either contribute to the brain damage owing to the development of oxidative stress, or might prevent cell death by the modulation of O\n                    <jats:sub>2<\/jats:sub>\n                    bioavailability to cells. Here, we studied hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) generation in mitochondria of neurons\n                    <jats:italic>in vivo<\/jats:italic>\n                    using the H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010sensitive biosensor HyPer7, and blood properties [haemoglobin (Hb) oxygenation and Hb affinity to O\n                    <jats:sub>2<\/jats:sub>\n                    , estimated with Raman microspectroscopy (RS)] in two experimental rat models: transient 60\u2010min ischaemia induced by the occlusion of the middle cerebral artery (MCAO) followed by 48\u2009h reperfusion, and permanent 48\u2010h MCAO\u2010induced ischaemia. We found that an increase in the amount of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    synthesised in neuronal mitochondria under the reperfusion period correlated with a decrease in blood oxygenation level, whereas permanent ischaemia did not affect the amount of oxyhaemoglobin but led to an increase in the affinity of Hb to O\n                    <jats:sub>2<\/jats:sub>\n                    . We hypothesise that H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    may initiate processes that lead to increased O\n                    <jats:sub>2<\/jats:sub>\n                    penetration to the brain tissue under reperfusion, and that increased Hb affinity to O\n                    <jats:sub>2<\/jats:sub>\n                    may be an adaptive reaction of the blood system to acute prolonged ischaemia.\n                  <\/jats:p>","DOI":"10.1111\/febs.70453","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T13:09:56Z","timestamp":1771506596000},"page":"4342-4357","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ischaemia\/reperfusion and permanent ischaemia differentially affect haemoglobin properties \u2013 Possible influence of oxidative stress and adaptation to acute hypoxia"],"prefix":"10.1111","volume":"293","author":[{"given":"Zhanna V.","family":"Bochkova","sequence":"first","affiliation":[{"name":"Faculty of Biology, M.V. Lomonosov Moscow State University  Russia"},{"name":"M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita A.","family":"Khlystova","sequence":"additional","affiliation":[{"name":"M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences  Moscow Russia"},{"name":"Federal Center of Brain Research and Neurotechnologies, Federal Medical Biological Agency  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika A.","family":"Katrukha","sequence":"additional","affiliation":[{"name":"M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences  Moscow Russia"},{"name":"Federal Center of Brain Research and Neurotechnologies, Federal Medical Biological Agency  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adil A.","family":"Baizhumanov","sequence":"additional","affiliation":[{"name":"Faculty of Biology, M.V. Lomonosov Moscow State University  Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina V.","family":"Vasilyeva","sequence":"additional","affiliation":[{"name":"M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasiya E.","family":"Nosova","sequence":"additional","affiliation":[{"name":"M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei M.","family":"Karhov","sequence":"additional","affiliation":[{"name":"M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeniia Y.","family":"Parshina","sequence":"additional","affiliation":[{"name":"Faculty of Biology, M.V. Lomonosov Moscow State University  Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxim A.","family":"Solotenkov","sequence":"additional","affiliation":[{"name":"Faculty of Physics, M.V. Lomonosov Moscow State University  Russia"},{"name":"Life Improvement by Future Technologies Center  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandr A.","family":"Moshchenko","sequence":"additional","affiliation":[{"name":"Federal Center of Brain Research and Neurotechnologies, Federal Medical Biological Agency  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kseniia I.","family":"Morozova","sequence":"additional","affiliation":[{"name":"Faculty of Biology, M.V. Lomonosov Moscow State University  Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir M.","family":"Kassem","sequence":"additional","affiliation":[{"name":"Faculty of Biology, M.V. Lomonosov Moscow State University  Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgy V.","family":"Maksimov","sequence":"additional","affiliation":[{"name":"Faculty of Biology, M.V. Lomonosov Moscow State University  Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir A.","family":"Oleinikov","sequence":"additional","affiliation":[{"name":"M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir A.","family":"Mitkevich","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander A.","family":"Makarov","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6637-8098","authenticated-orcid":false,"given":"Vsevolod V.","family":"Belousov","sequence":"additional","affiliation":[{"name":"M.M. Shemyakin and Yu. A. 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