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In glial cells, DAO metabolizes D\u2010serine, which acts as a co\u2010agonist and regulator of glutamate receptors. We previously reported that DAO is also expressed in epithelial cells of the choroid plexus (CP); however, the subcellular localization and functional significance of DAO in the CP remain unclear. In this study, we performed histological analyses to investigate the subcellular localization of DAO in the CP. We first confirmed DAO expression in the mouse CP. Immunostaining revealed vesicle\u2010like DAO signals in the cell bodies of choroid plexus epithelial cells (CPECs). Super\u2010resolution microscopy was then used to evaluate colocalization with intracellular organelle markers. DAO signals partially colocalized with ATP\u2010binding cassette subfamily D member 3 (PMP70) and peroxisomal targeting signal 1 receptor (PEX5). In contrast, they colocalized with Golgin subfamily A member 2 (GM130), trans\u2010Golgi network integral membrane protein 2 (TGN46), adaptor protein complex AP\u20102 (AP\u20102), early endosome antigen 1 (EEA1), Ras\u2010related proteins Rab5a, Rab11, CD63 antigen (CD63), tumor susceptibility gene 101 protein (TSG101), lysosome\u2010associated membrane glycoprotein 1 (LAMP\u20101), LAMP\u20102, beclin\u20101 and microtubule\u2010associated protein 1 light chain 3 (LC3). These findings suggest that, in the CP, DAO is transported from the Golgi apparatus to endosomes and subsequently distributed to multiple vesicular compartments. The presence of DAO in peroxisomes, autophagosomes, lysosomes, and exosomes indicated diverse intracellular localization within CPECs. This distribution may enable efficient metabolism of blood\u2010derived D\u2010serine in CPECs.<\/jats:p>","DOI":"10.1111\/febs.70459","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T12:53:27Z","timestamp":1771332807000},"page":"4358-4373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Variability in intracellular localization of D\u2010amino acid oxidase in choroid plexus epithelial cells"],"prefix":"10.1111","volume":"293","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5627-026X","authenticated-orcid":false,"given":"Koji","family":"Ono","sequence":"first","affiliation":[{"name":"School of Medical Technology, Faculty of Health and Medical Care, Saitama Medical University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Shishido","sequence":"additional","affiliation":[{"name":"Institute of Photonics and Human Health Frontier 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