{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T04:38:56Z","timestamp":1792471136440,"version":"4.5.0"},"reference-count":41,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT2016547"],"award-info":[{"award-number":["GNT2016547"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:p>\n                    Dynamin\u2010related protein 1 (Drp1), encoded by\n                    <jats:italic>DNM1L<\/jats:italic>\n                    , is essential for mitochondrial fission, but its functional roles remain unclear due to isoform\u2010specific effects from alternative splicing. Short\u2010read RNA sequencing fails to resolve full\u2010length isoforms involving distant exons, limiting our understanding. Here, we applied targeted long\u2010read sequencing to profile full\u2010length\n                    <jats:italic>DNM1L<\/jats:italic>\n                    transcripts in human left ventricle and induced pluripotent stem cell\u2010derived cardiomyocytes, recovering all annotated isoforms with conserved expression patterns and isoforms 1\u20134 being the most abundant. Functional assays revealed that isoform abundance does not predict enzymatic activity. Extending this to six different mouse tissues, we identified distinct, tissue\u2010enriched expression profiles. Functional rescue in Drp1\u2010knockout mouse embryonic fibroblasts showed isoform\u2010dependent differences in mitochondrial fission. Isoforms lacking the A\u2010insert robustly rescued mitochondrial fission, with maximal activity observed for variants also lacking the B\u2010insert (e.g. isoform b), consistent with a modulatory role of exon 3 in Drp1 activity. Our cross\u2010species atlas integrates long\u2010read transcriptomics with functional validation, revealing how isoform diversity underpins tissue\u2010specific mitochondrial dynamics and physiological roles of Drp1.\n                  <\/jats:p>","DOI":"10.1111\/febs.70568","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:44:21Z","timestamp":1777470261000},"page":"6208-6229","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Long\u2010read sequencing\u2010based atlas of tissue\u2010specific expression of\n                    <i>DNM1L<\/i>\n                    transcript variants"],"prefix":"10.1111","volume":"293","author":[{"given":"Feng","family":"Yan","sequence":"first","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research  Parkville Australia"},{"name":"Department of Medical Biology The University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jafar S.","family":"Jabbari","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research  Parkville Australia"},{"name":"Department of Medical Biology The University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi X. 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