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This sulfation can occur at the N\u2010terminal tyrosine residues (Y3, Y10, Y14, and Y15) of CCR5 and is catalyzed by human tyrosylprotein sulfotransferases (hTPSTs) within the Golgi lumen. However, the detailed molecular mechanism by which these tyrosine residues are sulfated remains unresolved. To elucidate the mechanism, we determined the crystal structure of a soluble domain of hTPST1 bound to the sulfate donor product 3\u2032\u2010phosphoadenosine 5\u2032\u2010phosphate (PAP) and a modified 18\u2010residue CCR5 peptide designed to isolate the Y3\u2010centered binding mode, at 3.2\u2009\u00c5 resolution, with six peptide residues ordered. This structure defines key interactions consistent with Y3 sulfation and is consistent with previous biochemical data. Based on the crystal structure and prior knowledge, we constructed peptide docking models for Y10, Y14, and Y15 sulfation, as well as full\u2010length hTPST1\u2010PAP\u2010CCR5 docking models. The crystal structure provides experimental insight into Y3 recognition, whereas the docking models provide testable hypotheses for how the other sulfation sites may be accommodated in the context of CCR5.<\/jats:p>","DOI":"10.1111\/febs.70597","type":"journal-article","created":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T06:20:51Z","timestamp":1779517251000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Structural insights into tyrosine sulfation of\n                    <scp>CCR5<\/scp>\n                    by human tyrosylprotein sulfotransferase\u20101"],"prefix":"10.1111","author":[{"given":"Shinnosuke","family":"Tanaka","sequence":"first","affiliation":[{"name":"Faculty of Agriculture, Laboratory of Biophysical Chemistry, Department of Bioscience and Biotechnology Kyushu University  Fukuoka 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