{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T22:40:15Z","timestamp":1793745615022,"version":"4.6.1"},"reference-count":78,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM146611"],"award-info":[{"award-number":["T32GM146611"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL159512"],"award-info":[{"award-number":["R01HL159512"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015326","name":"Common Fund","doi-asserted-by":"publisher","award":["U54CA272205"],"award-info":[{"award-number":["U54CA272205"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01NS121145"],"award-info":[{"award-number":["R01NS121145"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R21NS107941"],"award-info":[{"award-number":["R21NS107941"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"abstract":"<jats:p>\n                    Astrocytes, glial cells in the brain, play a crucial role in maintaining brain homeostasis through functional gap junctions (GJs) primarily comprising connexin43 (Cx43). These GJs facilitate electrical and metabolic coupling between astrocytes. Dysregulation of Cx43 has been implicated in various pathologies, including traumatic brain injury (TBI) and acquired epilepsy. After mild TBI\/concussion, we previously identified a subset of atypical astrocytes that are associated with the development of spontaneous seizures. These astrocytes exhibit reduced Cx43 expression and coupling. However, previous studies have suggested an overall increase in Cx43 protein after TBI. Cx43 also has non\u2010junctional and channel\u2010independent functions including hemichannel communication with the extracellular milieu. In the present study, we set out to determine how mild TBI initiates alterations to Cx43 protein expression and localization, how these alterations are regulated, and whether they contribute to seizure susceptibility. We demonstrate remarkable heterogeneity of Cx43 protein levels across individual astrocytes. A subset of astrocytes displayed decreased Cx43 expression, yet total cortical Cx43 protein increased. Subcellularly, junctional Cx43 remained stable, while hemichannels and\/or cytoplasmic Cx43 were increased. Phosphorylation of Cx43 at serine 368, a key regulatory site, increased after mild TBI. Critically, in Cx43\n                    <jats:sup>S368A<\/jats:sup>\n                    mutant mice lacking this phosphorylation, the mild TBI\u2010induced increase in soluble non\u2010junctional Cx43 and increased hemichannel function did not occur. Furthermore, Cx43\n                    <jats:sup>S368A<\/jats:sup>\n                    mutant mice exhibited reduced susceptibility to pentylenetetrazol\u2010induced seizures, suggesting that TBI\u2010induced Cx43\n                    <jats:sup>S368<\/jats:sup>\n                    phosphorylation enhances seizure susceptibility. We therefore anticipate that inhibiting this post\u2010translational modification may represent a potential therapeutic strategy to mitigate neuronal hyperexcitability and seizure development.\n                  <\/jats:p>","DOI":"10.1111\/febs.70609","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T12:41:04Z","timestamp":1780663264000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Astrocytic connexin43 phosphorylation contributes to seizure susceptibility after mild traumatic brain injury"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2298-7753","authenticated-orcid":false,"given":"Carmen","family":"Mu\u00f1oz\u2010Ballester","sequence":"first","affiliation":[{"name":"Cell, Developmental and Integrative Biology Department University of Alabama at Birmingham  Alabama USA"},{"name":"Department of Biological Sciences University of Maryland Baltimore County  Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Owen","family":"Leitzel","sequence":"additional","affiliation":[{"name":"Cell, Developmental and Integrative Biology Department University of Alabama at Birmingham  Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Golf","sequence":"additional","affiliation":[{"name":"Cell, Developmental and Integrative Biology Department University of Alabama at Birmingham  Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chelsea M.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Fralin Biomedical Research Institute at Virginia Tech Carilion  Roanoke Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Zeitz","sequence":"additional","affiliation":[{"name":"Fralin Biomedical Research Institute at Virginia Tech Carilion  Roanoke Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Pandit","sequence":"additional","affiliation":[{"name":"Cell, Developmental and Integrative Biology Department University of Alabama at Birmingham  Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Wash","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Maryland Baltimore County  Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenna V.","family":"Donohue","sequence":"additional","affiliation":[{"name":"Cell, Developmental and Integrative Biology Department University of Alabama at Birmingham  Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Istre","sequence":"additional","affiliation":[{"name":"Cell, Developmental and Integrative Biology Department University of Alabama at Birmingham  Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"Smyth","sequence":"additional","affiliation":[{"name":"Fralin Biomedical Research Institute at Virginia Tech Carilion  Roanoke Virginia USA"},{"name":"Department of Biological Sciences, College of Science Virginia Tech  Blacksburg Virginia USA"},{"name":"Virginia Tech Carilion School of Medicine  Roanoke Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2875-494X","authenticated-orcid":false,"given":"Samy","family":"Lamouille","sequence":"additional","affiliation":[{"name":"Fralin Biomedical Research Institute at Virginia Tech Carilion  Roanoke Virginia USA"},{"name":"Department of Biological Sciences, College of Science Virginia Tech  Blacksburg Virginia USA"},{"name":"Virginia Tech Carilion School of Medicine  Roanoke Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6716-3670","authenticated-orcid":false,"given":"Stefanie","family":"Robel","sequence":"additional","affiliation":[{"name":"Cell, Developmental and Integrative Biology Department University of Alabama at Birmingham  Alabama USA"},{"name":"Department of Physical Medicine and Rehabilitation University of Alabama at Birmingham  Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1126\/science.1164022","article-title":"Astroglial metabolic networks sustain hippocampal synaptic transmission","volume":"322","author":"Rouach N","year":"2008","journal-title":"Science"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2024.114504","article-title":"Astroglial networks control visual responses of 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