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Recent studies have linked increased ECF viscosity to the enhanced migratory capacity of cells across a wide range of environments. The mechanical properties of cells have been shown to regulate cell motility. As ECF viscosity is mechanical, the resulting increased cell migration due to ECF viscosity could be mediated by modulated cellular mechanoresponses. In this work, we varied extracellular fluid viscosity through addition of methylcellulose (MC) or polyvinylpyrrolidone (PVP) to the cell culture media. Cell morphology and migration parameters were quantified from phase\u2010contrast microscopy images and were measured as a function of extracellular fluid viscosity and\/or osmotic shocks. We also utilized a microfluidic cell deformation device and Brillouin microscopy to measure the mechanical properties of cells as a function of extracellular fluid viscosity. We demonstrated that increased ECF viscosity leads to changes in cell mechanical properties and invasive potential in a macromolecule\u2010dependent manner.<\/jats:p>","DOI":"10.1111\/febs.70623","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T05:25:33Z","timestamp":1781587533000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Extracellular fluid viscosity influences the mechanics and\u00a0migration of\n                    <scp>MDA<\/scp>\n                    \u2010\n                    <scp>MB<\/scp>\n                    \u2010231 cells in a macromolecule\u2010dependent manner"],"prefix":"10.1111","author":[{"given":"Ian M.","family":"Smith","sequence":"first","affiliation":[{"name":"Fischell Department of Bioengineering University of Maryland, College Park  MD 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