{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:50:14Z","timestamp":1787014214627,"version":"build-2736575974"},"reference-count":68,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T00:00:00Z","timestamp":1782000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T00:00:00Z","timestamp":1782000000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN 2017\u201006785"],"award-info":[{"award-number":["RGPIN 2017\u201006785"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN 2020\u201005274"],"award-info":[{"award-number":["RGPIN 2020\u201005274"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"abstract":"<jats:p>\n                    The concepts of physioxia and physiological media for mammalian cell culture have gained attention over the past several years. Although the effects of oxygen tension or nutrient composition have been examined individually, their combined, large\u2010scale impacts on cancer cell biology remain poorly understood. Here, we integrated transcriptomic, proteomic, and functional analyses to assess how oxygen levels (18% vs. 5% O\n                    <jats:sub>2<\/jats:sub>\n                    ) and medium composition (DMEM vs. Plasmax) influence human breast cancer (MCF7) cells. We found that culturing MCF7 cells in physioxia (5% O\n                    <jats:sub>2<\/jats:sub>\n                    ) and Plasmax medium induces a transcriptional profile that more closely resembles breast tumors\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Moreover, changes in transcript and protein abundance were significantly associated with cellular growth, motility, and metabolism. At the functional level, oxygen level and culture medium affected proliferation, migration, glucose consumption, and metabolic activity in MCF7 cells. We conclude that both oxygen levels and medium composition in culture modulate hallmark cancer phenotypes, underscoring the importance of mimicking physiological microenvironments when studying biological mechanisms and therapeutic approaches in cancer.\n                  <\/jats:p>","DOI":"10.1111\/febs.70629","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T04:46:08Z","timestamp":1782103568000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Physiological media and oxygen levels modulate cancer hallmarks in cultured breast cancer cells"],"prefix":"10.1111","author":[{"given":"Jacob E.","family":"Wiebe","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Brock University  St. Catharines Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9468-0080","authenticated-orcid":false,"given":"Ricardo","family":"Alva","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Brock University  St. Catharines Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fereshteh","family":"Moradi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Brock University  St. Catharines Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Alderman","sequence":"additional","affiliation":[{"name":"Department of Integrative Biology University of Guelph  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Brock University  St. Catharines Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5894-0300","authenticated-orcid":false,"given":"Jeffrey A.","family":"Stuart","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Brock University  St. Catharines Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1038\/nature00766","article-title":"Mutations of the BRAF gene in human cancer","volume":"417","author":"Davies H","year":"2002","journal-title":"Nature"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/nm0596-561","article-title":"Effects of a selective inhibitor of the Abl tyrosine kinase on the growth of Bcr\u2010Abl positive cells","volume":"2","author":"Druker B","year":"1996","journal-title":"Nat 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