{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T08:15:06Z","timestamp":1783325706925,"version":"3.54.6"},"reference-count":66,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["FAPESP grant 2021\/11784\u20109 to IM"],"award-info":[{"award-number":["FAPESP grant 2021\/11784\u20109 to IM"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["FAPESP grants 2008\/57566\u20108"],"award-info":[{"award-number":["FAPESP grants 2008\/57566\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/24705\u20103 to MCB"],"award-info":[{"award-number":["2013\/24705\u20103 to MCB"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"abstract":"<jats:p>\n                    The\n                    <jats:italic>Neurospora crassa<\/jats:italic>\n                    RVB\u20101 and RVB\u20102 proteins belong to the AAA+ (ATPases Associated with various cellular Activities) superfamily and are organized in a hetero\u2010hexameric complex essential for multiple cellular processes. Although they have been described as associated with cancer, new molecular functions have recently been described for them. Here, we investigate their participation in the fungus stress response, and our results support that they are components of the regulatory mechanism involved in the cellular stress response. Results of gene and protein expression, as well as cellular localization, revealed that their expression and location are modulated under stressing conditions, such as temperature, salt, and light. Furthermore, they are components of cytoplasmic granules induced by stress. Through immunoprecipitation assays followed by mass spectrometry, we identified many RVB\u20101 interactors, including components of chromatin remodeling complexes, some of which showed to be involved in stress response. New RVB\u20101 partners were identified, such as proteins participating in carbohydrate and cell wall metabolism, cell signaling, among other processes. One such new partner is shugoshin (SGO\u20101), a pericentromeric protein described as a regulator of chromosomal segregation during meiosis. Using genetic and biochemical approaches, we demonstrate the RVB\u20101 and SGO\u20101 interaction, and the structural aspects were analyzed by AlphaFold3. This interaction reveals a new RVB\u20101 function as a protein likely important for the maintenance of the centromeric region. Our findings confirm the relevant function of RVBs as stress sensors in\n                    <jats:italic>N. crassa<\/jats:italic>\n                    and reveal new interactors, highlighting their importance in the context of cellular metabolism.\n                  <\/jats:p>","DOI":"10.1111\/febs.70643","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T07:21:16Z","timestamp":1783322476000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>RVB<\/scp>\n                    \u20101 and\n                    <scp>RVB<\/scp>\n                    \u20102 are stress responsive proteins in\n                    <i>Neurospora crassa<\/i>\n                    and\n                    <scp>RVB<\/scp>\n                    \u20101 interacts with the centromeric Shugoshin (\n                    <scp>SGO<\/scp>\n                    \u20101) protein"],"prefix":"10.1111","author":[{"given":"Jonatas Erick Maimoni","family":"Campanella","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Qu\u00edmica Org\u00e2nica, Instituto de Qu\u00edmica Universidade Estadual Paulista, UNESP  Araraquara SP Brazil"},{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade Universidade Federal de S\u00e3o Carlos, UFSCar  S\u00e3o Carlos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Aboud","family":"Barbugli","sequence":"additional","affiliation":[{"name":"Departamento de Materiais Dent\u00e1rios e Pr\u00f3tese, Faculdade de Odontologia Universidade Estadual Paulista, UNESP  Araraquara SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoniel Augusto Severo","family":"Gomes","sequence":"additional","affiliation":[{"name":"CRM<sup>2<\/sup>, CNRS UMR 7036, Facult\u00e9 des Sciences et Technologies Universit\u00e9 de Lorraine  Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Zanolli","family":"Freitas","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Qu\u00edmica Org\u00e2nica, Instituto de Qu\u00edmica Universidade Estadual Paulista, UNESP  Araraquara SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iran","family":"Malavazi","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade Universidade Federal de S\u00e3o Carlos, UFSCar  S\u00e3o Carlos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5319-2710","authenticated-orcid":false,"given":"Maria C\u00e9lia","family":"Bertolini","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Qu\u00edmica Org\u00e2nica, Instituto de Qu\u00edmica Universidade 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