{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T09:19:23Z","timestamp":1783675163854,"version":"3.55.0"},"reference-count":64,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"abstract":"<jats:p>Laser microdissection\u2010based proteomics enables spatially resolved profiling of the proteome within intact tissue. Recent advances in histology, image analysis, laser microdissection and mass spectrometry have markedly increased the capability of this technology and driven the development of a range of powerful workflows. These now extend from regional tissue proteomics to emerging single\u2010cell applications, with subcellular proteome mapping on the horizon. In this review, we examine the current state of laser microdissection (LMD)\u2010based proteomics, outline what these approaches can offer, describe the standard workflow, and discuss which applications and instrumentation are best suited to address specific biological questions. We also highlight the strengths and limitations of current workflows and direct readers to key publications that describe and exemplify their successful implementation.<\/jats:p>","DOI":"10.1111\/febs.70653","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T09:00:49Z","timestamp":1783674049000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A guide to laser microdissection\u2010based proteomics"],"prefix":"10.1111","author":[{"given":"Cheng","family":"Shen","sequence":"first","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research  Parkville Australia"},{"name":"University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kael","family":"Schoffer","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research  Parkville Australia"},{"name":"University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Williams","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research  Parkville Australia"},{"name":"University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4657-9869","authenticated-orcid":false,"given":"Maria C.","family":"Tanzer","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research  Parkville Australia"},{"name":"University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","DOI":"10.1126\/science.aaa6090","article-title":"RNA imaging. Spatially resolved, highly multiplexed RNA profiling in single cells","volume":"348","author":"Chen KH","year":"2015","journal-title":"Science"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1126\/science.aaf2403","article-title":"Visualization and analysis of gene expression in tissue sections by spatial transcriptomics","volume":"353","author":"Stahl PL","year":"2016","journal-title":"Science"},{"key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1038\/s41374-020-0417-4","article-title":"Multiplexed ion beam imaging (MIBI) for characterization of the tumor microenvironment across tumor types","volume":"100","author":"Ptacek J","year":"2020","journal-title":"Lab Invest"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.aax5851","article-title":"MIBI\u2010TOF: a multiplexed imaging platform relates cellular phenotypes and tissue structure","volume":"5","author":"Keren L","year":"2019","journal-title":"Sci Adv"},{"key":"e_1_2_11_6_1","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1039\/D5AY01341K","article-title":"MALDI spatial proteomics: a mini review of approaches and techniques","volume":"18","author":"Lopez A","year":"2026","journal-title":"Anal Methods"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.202000077","article-title":"Microdissection\u2010an essential prerequisite for spatial cancer omics","volume":"20","author":"Eggeling F","year":"2020","journal-title":"Proteomics"},{"key":"e_1_2_11_8_1","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1126\/science.274.5289.998","article-title":"Laser capture microdissection","volume":"274","author":"Emmert\u2010Buck MR","year":"1996","journal-title":"Science"},{"key":"e_1_2_11_9_1","first-page":"475","article-title":"Laser capture microdissection in the genomic and proteomic era: targeting the genetic basis of cancer","volume":"1","author":"Domazet B","year":"2008","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_11_10_1","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1038\/s41592-024-02565-3","article-title":"Method of the year 2024: spatial proteomics","volume":"21","year":"2024","journal-title":"Nat Methods"},{"key":"e_1_2_11_11_1","doi-asserted-by":"crossref","first-page":"15656","DOI":"10.1021\/acs.analchem.3c02856","article-title":"Parallelized acquisition of orbitrap and astral analyzers enables high\u2010throughput quantitative analysis","volume":"95","author":"Stewart HI","year":"2023","journal-title":"Anal Chem"},{"key":"e_1_2_11_12_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2025.101504","article-title":"Higher\u2010throughput proteome profiling enabled by parallelized pre\u2010accumulation and optimized ion processing in the Orbitrap astral zoom mass spectrometer","volume":"25","author":"Guzman UH","year":"2026","journal-title":"Mol Cell Proteomics"},{"key":"e_1_2_11_13_1","doi-asserted-by":"crossref","DOI":"10.15252\/msb.202110798","article-title":"Ultra\u2010high sensitivity mass spectrometry quantifies single\u2010cell proteome changes upon perturbation","volume":"18","author":"Brunner AD","year":"2022","journal-title":"Mol Syst Biol"},{"key":"e_1_2_11_14_1","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1038\/s41587-022-01302-5","article-title":"Deep visual proteomics defines single\u2010cell identity and heterogeneity","volume":"40","author":"Mund A","year":"2022","journal-title":"Nat Biotechnol"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1016\/j.ccell.2025.06.004","article-title":"Spatial proteo\u2010transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression","volume":"43","author":"Schweizer L","year":"2025","journal-title":"Cancer Cell"},{"key":"e_1_2_11_16_1","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1038\/s41592-023-02007-6","article-title":"Spatial single\u2010cell mass spectrometry defines zonation of the hepatocyte proteome","volume":"20","author":"Rosenberger FA","year":"2023","journal-title":"Nat Methods"},{"key":"e_1_2_11_17_1","doi-asserted-by":"crossref","unstructured":"OliinykD HennebergL BardziukovaA ThielertM Kj\u00e6rgaardJ ZwiebelM VasconezS OellerM MetousisA UgurEet\u00a0al. (2025)nanoPhos enables ultra\u2010sensitive and cell\u2010type resolved spatial phosphoproteomics.bioRxiv.","DOI":"10.1101\/2025.05.29.656770"},{"key":"e_1_2_11_18_1","doi-asserted-by":"crossref","first-page":"9378","DOI":"10.1038\/s41467-024-53683-7","article-title":"Spatial proteomics of single cells and organelles on tissue slides using filter\u2010aided expansion proteomics","volume":"15","author":"Dong Z","year":"2024","journal-title":"Nat Commun"},{"key":"e_1_2_11_19_1","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1016\/j.cels.2023.10.003","article-title":"A framework for ultra\u2010low\u2010input spatial tissue proteomics","volume":"14","author":"Makhmut A","year":"2023","journal-title":"Cell Syst"},{"key":"e_1_2_11_20_1","doi-asserted-by":"crossref","DOI":"10.15252\/emmm.202317459","article-title":"Quantitative multiorgan proteomics of fatal COVID\u201019 uncovers tissue\u2010specific effects beyond inflammation","volume":"15","author":"Schweizer L","year":"2023","journal-title":"EMBO Mol Med"},{"key":"e_1_2_11_21_1","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1002\/path.5420","article-title":"A streamlined mass spectrometry\u2010based proteomics workflow for large\u2010scale FFPE tissue analysis","volume":"251","author":"Coscia F","year":"2020","journal-title":"J Pathol"},{"key":"e_1_2_11_22_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2025.101044","article-title":"High\u2010throughput proteomic and Phosphoproteomic analysis of formalin\u2010fixed paraffin\u2010embedded tissues","volume":"24","author":"Haines M","year":"2025","journal-title":"Mol Cell Proteomics"},{"key":"e_1_2_11_23_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s12014-025-09567-z","article-title":"Mass spectrometry\u2010based proteomics of FFPE tissues: progress, limitations, and clinical translation barriers","volume":"22","author":"AlHammadi SA","year":"2025","journal-title":"Clin Proteomics"},{"key":"e_1_2_11_24_1","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1038\/s44318-024-00289-w","article-title":"Towards routine proteome profiling of FFPE tissue: insights from a 1,220\u2010case pan\u2010cancer study","volume":"44","author":"T\u00fcshaus J","year":"2025","journal-title":"EMBO J"},{"key":"e_1_2_11_25_1","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.jgg.2023.07.011","article-title":"Laser capture microdissection for biomedical research: towards high\u2010throughput, multi\u2010omics, and single\u2010cell resolution","volume":"50","author":"Guo W","year":"2023","journal-title":"J Genet Genomics"},{"key":"e_1_2_11_26_1","doi-asserted-by":"crossref","unstructured":"ChenYD CheungCHY Hsiao\u2010JenC SieYD ChungCW HuangCK HuangCC ChongWM LiuYP LinYCet\u00a0al. (2023)Microscopy\u2010guided subcellular proteomic discovery by high\u2010speed ultra\u2010content photo\u2010biotinylation.bioRxiv.","DOI":"10.1101\/2023.12.27.573388"},{"key":"e_1_2_11_27_1","doi-asserted-by":"crossref","first-page":"16878","DOI":"10.1038\/s41598-017-17204-5","article-title":"QuPath: open source software for digital pathology image analysis","volume":"7","author":"Bankhead P","year":"2017","journal-title":"Sci Rep"},{"key":"e_1_2_11_28_1","unstructured":"MicrosystemsL.Commercial platforms such as Aivia AI Image Analysis Software (Leica Microsystems) can\u00a0assist region\u2010of\u2010interest selection in LMD workflows."},{"key":"e_1_2_11_29_1","doi-asserted-by":"crossref","DOI":"10.15252\/msb.202211503","article-title":"Robust dimethyl\u2010based multiplex\u2010DIA doubles single\u2010cell proteome depth via a reference channel","volume":"19","author":"Thielert M","year":"2023","journal-title":"Mol Syst Biol"},{"key":"e_1_2_11_30_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/s44320-025-00168-4","article-title":"Spatial proteomics of ovarian cancer precursors delineates early disease changes and drug targets","volume":"22","author":"Makhmut A","year":"2026","journal-title":"Mol Syst Biol"},{"key":"e_1_2_11_31_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2024.110620","article-title":"Spatial characterization and stratification of colorectal adenomas by deep visual proteomics","volume":"27","author":"Kabatnik S","year":"2024","journal-title":"iScience"},{"key":"e_1_2_11_32_1","doi-asserted-by":"crossref","DOI":"10.1186\/s12964-026-02720-7","article-title":"Spatial protein expression patterns across pathologically\u2010associated fibers revealed molecular specialization in inclusion body myositis","volume":"24","author":"Nijssen TI","year":"2026","journal-title":"Cell Commun Signal"},{"key":"e_1_2_11_33_1","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1016\/j.molcel.2024.12.023","article-title":"Deciphering functional tumor\u2010immune crosstalk through highly multiplexed imaging and deep visual proteomics","volume":"85","author":"Zheng X","year":"2025","journal-title":"Mol Cell"},{"key":"e_1_2_11_34_1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/978-1-0716-5154-4_7","article-title":"Immunofluorescence imaging\u2010guided laser microdissection for ultralow input spatial tissue proteomics","volume":"3015","author":"Fritzsche S","year":"2026","journal-title":"Methods Mol Biol"},{"key":"e_1_2_11_35_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/978-1-0716-5154-4_8","article-title":"Low\u2010input proteomics protocols for laser\u2010captured FFPE samples with Orbitrap astral DIA\u2010MS","volume":"3015","author":"Musiani D","year":"2026","journal-title":"Methods Mol Biol"},{"key":"e_1_2_11_36_1","doi-asserted-by":"crossref","first-page":"6891","DOI":"10.1021\/acsomega.4c09289","article-title":"Overlap of formalin\u2010fixed paraffin\u2010embedded and fresh\u2010frozen matched tissues for proteomics and Phosphoproteomics","volume":"10","author":"Humphries EM","year":"2025","journal-title":"ACS Omega"},{"key":"e_1_2_11_37_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2023.100643","article-title":"A standardized and reproducible workflow for membrane glass slides in routine histology and spatial proteomics","volume":"22","author":"Nordmann TM","year":"2023","journal-title":"Mol Cell Proteomics"},{"key":"e_1_2_11_38_1","unstructured":"NimoJ ValdesDS TrinhM PentimalliT SchallenbergS KlauschenF HerseF FlorianS RajewskyN&CosciaF(2025)OpenDVP: an experimental and computational framework for community\u2010empowered deep visual proteomics.bioRxiv."},{"key":"e_1_2_11_39_1","doi-asserted-by":"crossref","first-page":"2869","DOI":"10.1039\/D2LC00384H","article-title":"Hanging drop sample preparation improves sensitivity of spatial proteomics","volume":"22","author":"Kwon Y","year":"2022","journal-title":"Lab Chip"},{"key":"e_1_2_11_40_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2025.101489","article-title":"An ultrasensitive spatial tissue proteomics workflow exceeding 100 proteomes per day","volume":"25","author":"Klingeberg M","year":"2026","journal-title":"Mol Cell Proteomics"},{"key":"e_1_2_11_41_1","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1038\/s41592-025-02595-5","article-title":"Cellpose3: one\u2010click image restoration for improved cellular segmentation","volume":"22","author":"Stringer C","year":"2025","journal-title":"Nat Methods"},{"key":"e_1_2_11_42_1","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1038\/s41591-024-02838-6","article-title":"Generative models improve fairness of medical classifiers under distribution shifts","volume":"30","author":"Ktena I","year":"2024","journal-title":"Nat Med"},{"key":"e_1_2_11_43_1","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1038\/s41467-018-03367-w","article-title":"Nanodroplet processing platform for deep and quantitative proteome profiling of 10\u2010100 mammalian cells","volume":"9","author":"Zhu Y","year":"2018","journal-title":"Nat Commun"},{"key":"e_1_2_11_44_1","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1038\/s41586-024-08061-0","article-title":"Spatial proteomics identifies JAKi as treatment for a lethal skin disease","volume":"635","author":"Nordmann TM","year":"2024","journal-title":"Nature"},{"key":"e_1_2_11_45_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2024.100750","article-title":"An automated and fast sample preparation workflow for laser microdissection guided ultrasensitive proteomics","volume":"23","author":"Makhmut A","year":"2024","journal-title":"Mol Cell Proteomics"},{"key":"e_1_2_11_46_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2022.114924","article-title":"Maximizing hydrophobic peptide recovery in proteomics and antibody development using a mass spectrometry compatible surfactant","volume":"658","author":"Nie S","year":"2022","journal-title":"Anal Biochem"},{"key":"e_1_2_11_47_1","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1038\/s41586-025-08885-4","article-title":"Deep visual proteomics maps proteotoxicity in a genetic liver disease","volume":"642","author":"Rosenberger FA","year":"2025","journal-title":"Nature"},{"key":"e_1_2_11_48_1","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1038\/s41592-020-00998-0","article-title":"diaPASEF: parallel accumulation\u2010serial fragmentation combined with data\u2010independent acquisition","volume":"17","author":"Meier F","year":"2020","journal-title":"Nat Methods"},{"key":"e_1_2_11_49_1","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1038\/s42255-026-01459-2","article-title":"Single\u2010cell spatial proteomics maps human liver zonation patterns and their vulnerability to disruption in tissue architecture","volume":"8","author":"Weiss CAM","year":"2026","journal-title":"Nat Metab"},{"key":"e_1_2_11_50_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2022.100489","article-title":"Synchro\u2010PASEF allows precursor\u2010specific fragment ion extraction and interference removal in data\u2010independent acquisition","volume":"22","author":"Skowronek P","year":"2023","journal-title":"Mol Cell Proteomics"},{"key":"e_1_2_11_51_1","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1021\/acs.jproteome.4c00037","article-title":"Spatial proteomics reveals alcohol\u2010induced damages to the crypts and villi of the mouse small intestine","volume":"23","author":"Suresh PS","year":"2024","journal-title":"J Proteome Res"},{"key":"e_1_2_11_52_1","doi-asserted-by":"crossref","first-page":"7710","DOI":"10.1038\/s41467-023-43520-8","article-title":"Deep topographic proteomics of a human brain tumour","volume":"14","author":"Davis S","year":"2023","journal-title":"Nat Commun"},{"key":"e_1_2_11_53_1","doi-asserted-by":"crossref","first-page":"10482","DOI":"10.1038\/s41467-025-65477-6","article-title":"Integrated spatial proteomic analysis of breast cancer heterogeneity unravels cancer cell phenotypic plasticity","volume":"16","author":"Mardamshina M","year":"2025","journal-title":"Nat Commun"},{"key":"e_1_2_11_54_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2022.110955","article-title":"Spatial region\u2010resolved proteome map reveals mechanism of COVID\u201019\u2010associated heart injury","volume":"39","author":"Leng L","year":"2022","journal-title":"Cell Rep"},{"key":"e_1_2_11_55_1","doi-asserted-by":"crossref","first-page":"4012","DOI":"10.1038\/s41467-022-31659-9","article-title":"Spatially resolved proteomic map shows that extracellular matrix regulates epidermal growth","volume":"13","author":"Li J","year":"2022","journal-title":"Nat Commun"},{"key":"e_1_2_11_56_1","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1007\/s00011-023-01767-3","article-title":"Spatial proteomics landscape and immune signature analysis of renal sample of lupus nephritis based on laser\u2010captured microsection","volume":"72","author":"Zheng F","year":"2023","journal-title":"Inflamm Res"},{"key":"e_1_2_11_57_1","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1186\/s12931-025-03332-4","article-title":"Atypical mesenchyme in congenital pulmonary airways malformation: a promising new focus","volume":"26","author":"Azar P","year":"2025","journal-title":"Respir Res"},{"key":"e_1_2_11_58_1","doi-asserted-by":"crossref","DOI":"10.15252\/msb.20199243","article-title":"Systematic genetics and single\u2010cell imaging reveal widespread morphological pleiotropy and cell\u2010to\u2010cell variability","volume":"16","author":"Mattiazzi Usaj M","year":"2020","journal-title":"Mol Syst Biol"},{"key":"e_1_2_11_59_1","doi-asserted-by":"crossref","first-page":"2264","DOI":"10.1038\/s41592-025-02808-x","article-title":"Deep generative modeling of sample\u2010level heterogeneity in single\u2010cell genomics","volume":"22","author":"Boyeau P","year":"2025","journal-title":"Nat Methods"},{"key":"e_1_2_11_60_1","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1038\/s41592-024-02559-1","article-title":"Challenging the astral mass analyzer to quantify up to 5,300 proteins per single cell at unseen accuracy to uncover cellular heterogeneity","volume":"22","author":"Bubis JA","year":"2025","journal-title":"Nat Methods"},{"key":"e_1_2_11_61_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-026-71418-8","article-title":"Deep visual proteomics uncovers nociceptor diversity and pain targets","volume":"17","author":"Chakrabarti S","year":"2026","journal-title":"Nat Commun"},{"key":"e_1_2_11_62_1","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1038\/nbt.3327","article-title":"High\u2010throughput phosphoproteomics reveals in vivo\u00a0insulin signaling dynamics","volume":"33","author":"Humphrey SJ","year":"2015","journal-title":"Nat Biotechnol"},{"key":"e_1_2_11_63_1","doi-asserted-by":"crossref","first-page":"4179","DOI":"10.1021\/acs.jproteome.0c00354","article-title":"Phosphorylation ratio determination in fresh\u2010frozen and formalin\u2010fixed paraffin\u2010embedded tissue with targeted mass spectrometry","volume":"19","author":"Zeneyedpour L","year":"2020","journal-title":"J Proteome Res"},{"key":"e_1_2_11_64_1","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/s43586-024-00318-2","article-title":"Top\u2010down proteomics","volume":"4","author":"Roberts DS","year":"2024","journal-title":"Nat Rev Methods Primers"},{"key":"e_1_2_11_65_1","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1038\/s41467-025-56523-4","article-title":"Spatial integration of multi\u2010omics single\u2010cell data with SIMO","volume":"16","author":"Yang P","year":"2025","journal-title":"Nat Commun"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.70653","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/febs.70653","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.70653","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T09:00:56Z","timestamp":1783674056000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.70653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,10]]},"references-count":64,"alternative-id":["10.1111\/febs.70653"],"URL":"https:\/\/doi.org\/10.1111\/febs.70653","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,10]]},"assertion":[{"value":"2026-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"febs.70653"}}