{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T06:30:59Z","timestamp":1787121059545,"version":"build-2736575974"},"reference-count":63,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"abstract":"<jats:p>\n                    Fungal phytopathogens represent a major threat to global agriculture, causing extensive yield losses. Among them,\n                    <jats:italic>Colletotrichum<\/jats:italic>\n                    spp. are infamous for their capacity of infecting a wide variety of monocot and dicot hosts. Motivated by the recent discovery in\n                    <jats:italic>Colletotrichum orbiculare<\/jats:italic>\n                    of a redox relay between Copper Radical Alcohol Oxidases from the AA5_2 subfamily and tandem peroxidases involved in plant infection, we investigated species from the\n                    <jats:italic>Colletotrichum<\/jats:italic>\n                    species complex (\n                    <jats:italic>C. acutatum<\/jats:italic>\n                    and\n                    <jats:italic>C. tamarilloi<\/jats:italic>\n                    ) where the tandem peroxidase gene is absent. Instead, putative ring\u2010cleavage dioxygenases are found within a conserved genomic locus adjacent to AA5_2 paralogues. Therefore, we hypothesized that ring\u2010cleavage dioxygenases could function as redox partners for AA5_2. Given the limited information available on fungal dioxygenases, we performed a biochemical characterization of both\n                    <jats:italic>Colletotrichum<\/jats:italic>\n                    ring\u2010cleavage dioxygenases. LC\u2013MS analyses revealed that both enzymes catalyze intradiol (1,2\u2010) ring cleavage of catecholic substrates and preferentially oxidize methyl\u2010substituted catechols. Structural modeling indicated an open, solvent\u2010exposed active site resembling that of spider mite intradiol ring cleavage dioxygenases and distinct from the compact, oligomeric bacterial homologs. However, both dioxygenases showed only a limited capacity to activate AA5_2\n                    <jats:italic>in vitro,<\/jats:italic>\n                    indicating that close genomic proximity does not necessarily imply efficient functional coupling. Together, these biochemical insights into both AA5_2 and ring\u2010cleavage dioxygenases provide a foundation for future studies aimed at elucidating their biological roles in fungal metabolism and pathogenicity.\n                  <\/jats:p>","DOI":"10.1111\/febs.70696","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T06:21:26Z","timestamp":1787120486000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Insights into tandem dioxygenases\u2014copper radical oxidases in fungal Phytopathogens of the\n                    <i>Colletotrichum acutatum<\/i>\n                    species complex"],"prefix":"10.1111","author":[{"given":"Radka","family":"Kon\u010dit\u00edkov\u00e1","sequence":"first","affiliation":[{"name":"Aix\u2010Marseille University, INRAE, BBF, Biodiversit\u00e9 et Biotechnologie Fongiques  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Ribeaucourt","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille University, INRAE, BBF, Biodiversit\u00e9 et Biotechnologie Fongiques  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireille","family":"Haon","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille University, INRAE, BBF, Biodiversit\u00e9 et Biotechnologie Fongiques  France"},{"name":"Aix Marseille University, INRAE, 3PE  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Navarro","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille University, INRAE, BBF, Biodiversit\u00e9 et Biotechnologie Fongiques  France"},{"name":"Aix Marseille University, INRAE, CIRM\u2010CF  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Drula","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille University, INRAE, BBF, Biodiversit\u00e9 et Biotechnologie Fongiques  France"},{"name":"Aix Marseille University, CNRS, UMR 7257 AFMB  France"},{"name":"INRAE, USC 1408 AFMB  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8354-3892","authenticated-orcid":false,"given":"Bastien","family":"Bissaro","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille University, INRAE, BBF, Biodiversit\u00e9 et Biotechnologie Fongiques  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7570-3745","authenticated-orcid":false,"given":"Jean\u2010Guy","family":"Berrin","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille University, INRAE, BBF, Biodiversit\u00e9 et Biotechnologie Fongiques  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2646-0286","authenticated-orcid":false,"given":"Mickael","family":"Lafond","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille University, INRAE, BBF, Biodiversit\u00e9 et Biotechnologie Fongiques  France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1126\/science.aap7999","article-title":"Worldwide emergence of resistance to antifungal drugs challenges human health and food security","volume":"360","author":"Fisher MC","year":"2018","journal-title":"Science"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3000302","article-title":"Plant health emergencies demand open science: tackling a cereal killer on the run","volume":"17","author":"Kamoun 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