{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:25:34Z","timestamp":1787041534371,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"abstract":"<jats:p>The Envelope (E) protein, one of the structural proteins encoded by coronavirus (CoV) genome, is involved in multiple processes of the virus life cycle and directly related to the pathogenesis process. The C\u2010terminus of E\u00a0contains an important virulence factor, a PDZ domain binding motif (PBM), whose deletion or modification is sufficient to attenuate the virus. Notably, the PBM of E interacts with the second PDZ domain (PDZ2) of host Zonula Occludens 1 protein (ZO1), leading to delayed formation of tight junctions (TJs) and disruption of the respiratory epithelium. Although the binding reaction between ZO1\u2010PDZ2 and E has been extensively studied since COVID\u201019 pandemic breakout, the structural determinants governing the binding process of ZO1\u2010PDZ2 by E protein are still poorly understood. In this scenario, we performed a comparative study through a combination of experimental NMR and computational methods to structurally characterize the interaction occurring between ZO1\u2010PDZ2 and three peptides mimicking the C\u2010terminal PBM of E protein from SARS\u2010CoV (now SARS\u2010CoV\u20101), SARS\u2010CoV\u20102, and MERS\u2010CoV. Our results could provide the basis for the development of therapeutic strategies that may reduce cell damage and the morbidity and mortality associated with coronavirus infection.<\/jats:p>","DOI":"10.1111\/febs.70699","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T07:45:55Z","timestamp":1787039155000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Insights into the molecular recognition of the second\n                    <scp>PDZ<\/scp>\n                    domain of\n                    <scp>ZO1<\/scp>\n                    by coronavirus Envelope\u2010derived peptides"],"prefix":"10.1111","author":[{"given":"Angelo","family":"Toto","sequence":"first","affiliation":[{"name":"Department of Biochemical Sciences Sapienza University of Rome  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Pennacchietti","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences Sapienza University of Rome  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Isernia","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies University of Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Caldarelli","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences Sapienza University of Rome  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaetano","family":"Malgieri","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies University of Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1653-1925","authenticated-orcid":false,"given":"Stefano","family":"Gianni","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences Sapienza University of Rome  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies University of Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3539-5343","authenticated-orcid":false,"given":"Roberto","family":"Fattorusso","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies University of Campania \u201cLuigi Vanvitelli\u201d  Caserta 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