{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T04:07:30Z","timestamp":1785902850883,"version":"3.56.0"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T00:00:00Z","timestamp":1473120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371475"],"award-info":[{"award-number":["81371475"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Brain and Behavior"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>Single nucleotide polymorphisms (<jats:styled-content style=\"fixed-case\">SNPs<\/jats:styled-content>) in the human type A gamma\u2010aminobutyric acid (<jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content>) receptor \u03b2<jats:sub>2<\/jats:sub> subunit gene (<jats:italic><jats:styled-content style=\"fixed-case\">GABRB2<\/jats:styled-content><\/jats:italic>) have been associated with schizophrenia and quantitatively correlated with <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in the postmortem brain tissue of patients with schizophrenia. <jats:sc>l<\/jats:sc>\u2010Methionine (<jats:styled-content style=\"fixed-case\">MET<\/jats:styled-content>) administration has been reported to cause a recrudescence of psychotic symptoms in patients with schizophrenia, and similar symptoms have been generated in <jats:styled-content style=\"fixed-case\">MET<\/jats:styled-content>\u2010induced mice. In this study, a zebrafish animal model was used to evaluate the relationship between the <jats:italic>gabrb2<\/jats:italic> <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression and its promoter <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation in developmental and <jats:styled-content style=\"fixed-case\">MET<\/jats:styled-content>\u2010induced schizophrenia\u2010like zebrafish. The results indicated developmental increases in global <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation and decreases in <jats:italic>gabrb2<\/jats:italic> promoter methylation in zebrafish. A significant increase in <jats:italic>gabrb2<\/jats:italic> <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels was observed after <jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content> was synthesized. Additionally, the <jats:styled-content style=\"fixed-case\">MET<\/jats:styled-content>\u2010triggered schizophrenia\u2010like symptoms in adult zebrafish, involving social withdrawal and cognitive dysfunction analyzed with social interaction and T\u2010maze behavioral tests, were accompanied by significantly increased <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation levels in the global genome and the <jats:italic>gabrb2<\/jats:italic> promoter. Furthermore, the significant correlation between <jats:italic>gabrb2<\/jats:italic> <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression and <jats:italic>gabrb2<\/jats:italic> promoter methylation observed in the developmental stages became non\u2010significant in <jats:styled-content style=\"fixed-case\">MET<\/jats:styled-content>\u2010triggered adult zebrafish. These findings demonstrate that <jats:italic>gabrb2<\/jats:italic> <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression is associated with <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation varies by developmental stage and show that these epigenetic association mechanisms are disrupted in <jats:styled-content style=\"fixed-case\">MET<\/jats:styled-content>\u2010triggered adult zebrafish with schizophrenia\u2010like symptoms. In conclusion, these results provide plausible epigenetic evidence of the <jats:styled-content style=\"fixed-case\">GABA<jats:sub>A<\/jats:sub><\/jats:styled-content> receptor \u03b2<jats:sub>2<\/jats:sub> subunit involvement in the schizophrenia\u2010like behaviors and demonstrate the potential use of zebrafish models in neuropsychiatric research.<\/jats:p>","DOI":"10.1111\/gbb.12315","type":"journal-article","created":{"date-parts":[[2016,8,10]],"date-time":"2016-08-10T12:59:50Z","timestamp":1470833990000},"page":"702-710","source":"Crossref","is-referenced-by-count":26,"title":["<scp>DNA<\/scp> methylation regulates <i>gabrb2<\/i><scp>mRNA<\/scp> expression: developmental variations and disruptions in <scp>l<\/scp>\u2010methionine\u2010induced zebrafish with schizophrenia\u2010like symptoms"],"prefix":"10.1111","volume":"15","author":[{"given":"L.","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Medical Genetics, School of Basic Medical Sciences Southern 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