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So far, the functions of Zfp462 remain unclear. In our study, we showed that Zfp462 is expressed predominantly in the developing brain, especially in the cerebral cortex and hippocampus regions from embryonic day 7.5 to early postnatal stage. By using a <jats:styled-content style=\"fixed-case\">piggyBac<\/jats:styled-content> transposon\u2010generated Zfp462 knockout (<jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content>) mouse model, we found that Zfp462 <jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> mice exhibited prenatal lethality with normal neural tube patterning, whereas heterozygous (Het) Zfp462 <jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> (Zfp462<jats:sup>+\/\u2212<\/jats:sup>) mice showed developmental delay with low body weight and brain weight. Behavioral studies showed that Zfp462<jats:sup>+\/\u2212<\/jats:sup> mice presented anxiety\u2010like behaviors with excessive self\u2010grooming and hair loss, which were similar to the pathological grooming behaviors in Hoxb8 <jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> mice. Further analysis of grooming microstructure showed the impairment of grooming patterning in Zfp462<jats:sup>+\/\u2212<\/jats:sup> mice. In addition, the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of <jats:italic>Pbx1<\/jats:italic> (pre\u2010B\u2010cell leukemia homeobox 1, an interacting protein of Zfp462) and <jats:italic>Hoxb8<\/jats:italic> decreased in the brains of Zfp462<jats:sup>+\/\u2212<\/jats:sup> mice, which may be the cause of anxiety\u2010like behaviors. Finally, imipramine, a widely used and effective anti\u2010anxiety medicine, rescued anxiety\u2010like behaviors and excessive self\u2010grooming in Zfp462<jats:sup>+\/\u2212<\/jats:sup> mice. In conclusion, Zfp462 deficiency causes anxiety\u2010like behaviors with excessive self\u2010grooming in mice. This provides a novel genetic mouse model for anxiety disorders and a useful tool to determine potential therapeutic targets for anxiety disorders and screen anti\u2010anxiety drugs.<\/jats:p>","DOI":"10.1111\/gbb.12339","type":"journal-article","created":{"date-parts":[[2016,9,13]],"date-time":"2016-09-13T08:32:40Z","timestamp":1473755560000},"page":"296-307","source":"Crossref","is-referenced-by-count":46,"title":["Zfp462 deficiency causes anxiety\u2010like behaviors with excessive self\u2010grooming in mice"],"prefix":"10.1111","volume":"16","author":[{"given":"B.","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurology The Second Affiliated Hospital of Soochow University  Shanghai China"},{"name":"Institute for Fetology The First Affiliated Hospital of Soochow University  Shanghai China"},{"name":"Institute of Neuroscience Soochow University  Shanghai 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