{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T19:46:31Z","timestamp":1775245591806,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T00:00:00Z","timestamp":1624838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes Brain and Behavior"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The genetic mechanisms of postoperative nausea and vomiting (PONV) and the involvement of the catecholamine system in the brain have not been elucidated. Eating kaolin clay as a type of pica has been examined as an alternative behavior to emesis. Here, we evaluated changes in whole\u2010transcriptome analysis in the nucleus of the solitary tract (NTS) in a rat pica model as a surrogate behavior of PONV to elucidate the molecular genetic mechanisms of the development of PONV and the involvement of the catecholamine system in the NTS. First, kaolin pica behaviors were investigated in 71 female Wistar rats following isoflurane anesthesia, surgical insult or morphine administration. Multiple linear regression analysis showed that 3\u00a0mg\/kg morphine increased kaolin intake by 2.8\u00a0g (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0002). Next, total RNA and protein were extracted from the dissected NTS, and whole\u2010transcriptome sequencing (RNA\u2010seq) was performed to identify PONV\u2010associated genes and to verify the involvement of the catecholamine system. The gene expression levels of tyrosine hydroxylase and dopamine beta\u2010hydroxylase in the catecholamine biosynthesis pathway decreased significantly in the PONV model. Release of noradrenaline, a catecholamine pathway end product, may have increased at the synaptic terminal of the NTS neuron after pica behavior. Systematic administration of \u03b12 adrenergic receptor agonists after surgery reduced kaolin intake from 3.2\u00a0g (control) to 1.0\u00a0g (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0014). These results indicated that catecholamine neurotransmission was involved in the development of PONV in the NTS.<\/jats:p>","DOI":"10.1111\/gbb.12759","type":"journal-article","created":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T07:35:36Z","timestamp":1623396936000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Crucial involvement of catecholamine neurotransmission in postoperative nausea and vomiting: Whole\u2010transcriptome profiling in the rat nucleus of the solitary tract"],"prefix":"10.1111","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5748-6302","authenticated-orcid":false,"given":"Shigekazu","family":"Sugino","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Tohoku University School of Medicine  Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Konno","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Tohoku University School of Medicine  Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junko","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Tohoku University School of Medicine  Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuka","family":"Imamura\u2010Kawasawa","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Department of Biochemistry and Molecular Biology Institute for Personalized Medicine, Pennsylvania State University College of Medicine  Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanta","family":"Kido","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Kanagawa Dental University Graduate School of Dentistry  Yokosuka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Tohoku University School of Medicine  Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Endo","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Tohoku University School of Medicine  Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Yamauchi","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine Tohoku University School of Medicine  Sendai 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