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In response to semaphorin axon guidance proteins, the MICAL1 protein is stimulated to produce reactive oxygen species that oxidize actin on specific methionine residues, leading to filamentous actin depolymerization and consequent changes in neuronal growth cone dynamics. Crossing genetically modified mice homozygous for floxed <jats:italic>Mical1<\/jats:italic> (<jats:italic>Mical1<\/jats:italic><jats:sup><jats:italic>fl\/fl<\/jats:italic><\/jats:sup>) alleles with transgenic mice expressing Cre recombinase under the control of a <jats:italic>tyrosinase<\/jats:italic> gene enhancer\/promoter (<jats:italic>Tyr::Cre<\/jats:italic>) enabled conditional <jats:italic>Mical1<\/jats:italic> deletion. Immunohistochemical analysis showed <jats:italic>Mical1<\/jats:italic> expression in the cerebellum, which plays a prominent role in the coordination of motor movements, with reduced <jats:italic>Mical1<\/jats:italic> expression in <jats:italic>Mical1<\/jats:italic><jats:sup><jats:italic>fl\/fl<\/jats:italic><\/jats:sup> mice co\u2010expressing <jats:italic>Tyr::Cre<\/jats:italic>. Analysis of the gaits of mice running on a treadmill showed that both male and female <jats:italic>Mical1<\/jats:italic><jats:sup><jats:italic>fl\/fl<\/jats:italic><\/jats:sup>, <jats:italic>Tyr::Cre<\/jats:italic> mutant mice had significant alterations to their striding patterns relative to wild\u2010type mice, although the specific aspects of their altered gaits differed between the sexes. Additional motor tests that involved movement on a rotating rod, descending a vertical pole, or crossing a balance beam did not show significant differences between the genotypes, suggesting that the effect of the <jats:italic>Mical1<\/jats:italic><jats:sup><jats:italic>fl\/fl<\/jats:italic><\/jats:sup>, <jats:italic>Tyr::Cre<\/jats:italic> genetic modifications was only manifested during specific highly coordinated movements that contribute to running. These findings indicate that there is a behavioral consequence in <jats:italic>Mical1<\/jats:italic><jats:sup><jats:italic>fl\/fl<\/jats:italic><\/jats:sup>, <jats:italic>Tyr::Cre<\/jats:italic> mutant mice that affects motor control as manifested by alterations in their gait.<\/jats:p>","DOI":"10.1111\/gbb.70004","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T11:24:30Z","timestamp":1727695470000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Mical1<\/i> deletion in <i>tyrosinase<\/i> expressing cells affects mouse running gaits"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5717-1121","authenticated-orcid":false,"given":"Katarina","family":"Micovic","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biology Toronto Metropolitan University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Canuel","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aasiya","family":"Remtulla","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Chuyen","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biology Toronto Metropolitan University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Byrsan","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Program Toronto Metropolitan University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"McGarry","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biology Toronto Metropolitan University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3428-3507","authenticated-orcid":false,"given":"Michael F.","family":"Olson","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biology Toronto Metropolitan University  Toronto Ontario Canada"},{"name":"Department of Pharmacology and Toxicology University of Toronto  Toronto Ontario Canada"},{"name":"Biomedical Engineering Program Toronto Metropolitan University  Toronto Ontario 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