{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T14:34:07Z","timestamp":1782484447062,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes Brain and Behavior"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cannabis use is a growing public health concern due to its neuropsychiatric consequences and potential epigenetic effects. This study investigated the methylation status of the\n                    <jats:italic>DAT1<\/jats:italic>\n                    dopamine transporter gene in individuals with cannabis use disorder (CUD) and examined associations with personality traits. A total of 490 male participants (212 with CUD and 278 controls) were assessed using the NEO Five\u2010Factor Inventory (NEO\u2010FFI) and the State\u2013Trait Anxiety Inventory (STAI). DNA methylation levels at 33 CpG sites within the\n                    <jats:italic>DAT1<\/jats:italic>\n                    promoter region were quantified through methylation\u2010specific PCR and sequencing. Although no significant group differences were found in overall methylation levels, logistic regression revealed significant associations between methylation status and personality dimensions. CUD was linked to higher neuroticism and openness, lower agreeableness and conscientiousness, and elevated anxiety scores. Importantly, reduced\n                    <jats:italic>DAT1<\/jats:italic>\n                    methylation was a significant predictor of CUD. These findings underscore the interplay between personality, gene regulation, and addiction, supporting a role for epigenetic mechanisms in the development and maintenance of substance use disorders. Future research should explore site\u2010specific CpG alterations and their longitudinal impact on neurobehavioral outcomes.\n                  <\/jats:p>","DOI":"10.1111\/gbb.70040","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T12:49:19Z","timestamp":1765975759000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Methylation Status of the\n                    <scp>DAT1<\/scp>\n                    Dopamine Transporter Gene in Individuals With Cannabis Use Disorder: Associations With Personality Traits"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7137-1429","authenticated-orcid":false,"given":"Aleksandra","family":"Suchanecka","sequence":"first","affiliation":[{"name":"Independent Laboratory of Behavioral Genetics and Epigenetics Pomeranian Medical University in Szczecin  Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7841-821X","authenticated-orcid":false,"given":"Remigiusz","family":"Rec\u0142aw","sequence":"additional","affiliation":[{"name":"Independent Laboratory of Behavioral Genetics and Epigenetics Pomeranian Medical University in Szczecin  Szczecin Poland"},{"name":"Department of Medical Sciences and Public Health Gdansk University of Physical Education and Sport  Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4254-5466","authenticated-orcid":false,"given":"Krzysztof","family":"Chmielowiec","sequence":"additional","affiliation":[{"name":"Department of Hygiene and Epidemiology, Collegium Medicum University of Zielona G\u00f3ra  Zielona G\u00f3ra Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3285-5313","authenticated-orcid":false,"given":"Jolanta","family":"Chmielowiec","sequence":"additional","affiliation":[{"name":"Department of Nursing, Collegium Medicum University of Zielona G\u00f3ra  Zielona G\u00f3ra Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-5838","authenticated-orcid":false,"given":"Jolanta","family":"Masiak","sequence":"additional","affiliation":[{"name":"Second Department of Psychiatry and Psychiatric Rehabilitation Medical University of Lublin  Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2633-520X","authenticated-orcid":false,"given":"Anna","family":"Grzywacz","sequence":"additional","affiliation":[{"name":"Independent Laboratory of Behavioral Genetics and Epigenetics Pomeranian Medical University in Szczecin  Szczecin 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