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Reconstructing microbial metabolic activity rates in these regions has been challenging, due to the technical difficulty of direct rate measurements. In Cariaco Basin, which is the largest permanently anoxic marine basin and an important model system for geobiology, long\u2010term monitoring has yielded time series for the concentrations of biologically important compounds; however, the underlying metabolite fluxes remain poorly quantified. Here, we present a computational approach for reconstructing vertical fluxes and in situ net production\/consumption rates from chemical concentration data, based on a 1\u2010dimensional time\u2010dependent diffusive transport model that includes adaptive penalization of overfitting. We use this approach to estimate spatiotemporally resolved fluxes of oxygen, nitrate, hydrogen sulfide, ammonium, methane, and phosphate within the sub\u2010euphotic Cariaco Basin water column (depths 150\u2013900\u00a0m, years 2001\u20132014) and to identify hotspots of microbial chemolithotrophic activity. Predictions of the fitted models are in excellent agreement with the data and substantially expand our knowledge of the geobiology in Cariaco Basin. In particular, we find that the diffusivity, and consequently fluxes of major reductants such as hydrogen sulfide, and methane, is about two orders of magnitude greater than previously estimated, thus resolving a long\u2010standing apparent conundrum between electron donor fluxes and measured dark carbon assimilation rates.<\/jats:p>","DOI":"10.1111\/gbi.12357","type":"journal-article","created":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T01:15:22Z","timestamp":1567991722000},"page":"628-642","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Microbial metabolite fluxes in a model marine anoxic ecosystem"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9216-5619","authenticated-orcid":false,"given":"Stilianos","family":"Louca","sequence":"first","affiliation":[{"name":"Institute of Ecology and Evolution University of Oregon Eugene OR USA"},{"name":"Department of Biology University of Oregon Eugene OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yrene M.","family":"Astor","sequence":"additional","affiliation":[{"name":"Estaci\u00f3n de Investigaciones Marinas de Margarita Fundaci\u00f3n La Salle de Ciencias Naturales Punta de Piedras Venezuela"},{"name":"Institute for Marine Remote Sensing University of South Florida Tampa FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Doebeli","sequence":"additional","affiliation":[{"name":"Biodiversity Research Centre University of British Columbia Vancouver British Columbia Canada"},{"name":"Department of Zoology University of British Columbia Vancouver British Columbia Canada"},{"name":"Department of Mathematics University of British Columbia Vancouver British Columbia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordon T.","family":"Taylor","sequence":"additional","affiliation":[{"name":"School of Marine and Atmospheric Sciences Stony Brook University New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary I.","family":"Scranton","sequence":"additional","affiliation":[{"name":"School of Marine and Atmospheric Sciences Stony Brook University New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,8]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"515","article-title":"Effects of variations in eddy diffusivity on property distributions in the oceans","volume":"37","author":"Armi L.","year":"1979","journal-title":"Journal of Marine Research"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err417"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02473-10"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1998.43.7.1500"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1137\/1.9781611971484"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam7240"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1196889"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame01817"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gca.2010.09.017"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420034790"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2003.48.4.1521"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00257"},{"key":"e_1_2_11_14_1","volume-title":"Hydrographic investigations in the Oslofjord: A study of water circulation and exchange processes","author":"Gade H. 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