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This unit mainly represents offshore\u2010to\u2010shelf environments affected by storms and density currents. The Los Molles Formation is one of the oldest source rocks in the Neuqu\u00e9n Basin and constitutes an unconventional shale gas reservoir of great economic importance. The aim of this work was to identify the microbial activity from the description and interpretation of microbially induced sedimentary structures (MISS), to determine the paleoenvironmental and paleoecological conditions under which they formed, and to establish a possible relationship between these structures and the trace fossil <jats:italic>Trichichnus<\/jats:italic>. Samples from the levels with MISS were analyzed and described from macroscopic and binocular observations, petrographic microscope thin sections, and SEM samples with EDS analyses. The results showed several levels of microbial mats presenting diverse MISS, including biolaminations and <jats:italic>Kinneyia<\/jats:italic>\u2010like wrinkles structures that were described at the macroscopic level. In thin sections, biolaminations, filament\u2010like microstructures with different degrees of development, oriented grains and pyrite were observed. SEM images and EDS analyses showed different types of filaments, coccoids and EPS with high concentrations of carbon. These results revealed that the studied levels fulfill the established biogenicity criteria, guaranteeing that they have a bacterial origin. The abundance of the trace fossil <jats:italic>Trichichnus<\/jats:italic> sp. throughout the section and the proximity to some <jats:italic>Kinneyia\u2010<\/jats:italic>like wrinkle structures levels suggests that the same organisms may have generated them. Furthermore, they revealed that the Los Molles Formation, at the time of its deposition, experienced paleoecological and paleoenvironmental conditions appropriate for the establishment and development of microbial mats. The extensive levels of microbial mats in the study area suggest that they may have been a source of organic matter for the generation of hydrocarbons from the Los Molles Formation.<\/jats:p>","DOI":"10.1111\/gbi.12580","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T00:03:17Z","timestamp":1700784197000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Microbial mats and their palaeoenvironmental analysis in offshore \u2013 shelf facies of the Los Molles Formation (Toarcian \u2013 Lower Callovian) in the Chacay Melehue area, Neuqu\u00e9n Basin, Argentina"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0286-0111","authenticated-orcid":false,"given":"Maximiliano Nicol\u00e1s","family":"Rodriguez","sequence":"first","affiliation":[{"name":"Universidad Nacional de R\u00edo Negro, Instituto de Investigaci\u00f3n en Paleobiolog\u00eda y Geolog\u00eda  R\u00edo Negro Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Investigaci\u00f3n en Paleobiolog\u00eda y Geolog\u00eda  R\u00edo Negro Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9bora Mical","family":"Campetella","sequence":"additional","affiliation":[{"name":"Universidad Nacional de R\u00edo Negro, Instituto de Investigaci\u00f3n en Paleobiolog\u00eda y Geolog\u00eda  R\u00edo Negro Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Investigaci\u00f3n en Paleobiolog\u00eda y Geolog\u00eda  R\u00edo Negro Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noelia Beatriz","family":"Carmona","sequence":"additional","affiliation":[{"name":"Universidad Nacional de R\u00edo Negro, Instituto de Investigaci\u00f3n en Paleobiolog\u00eda y Geolog\u00eda  R\u00edo Negro Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Investigaci\u00f3n en Paleobiolog\u00eda y Geolog\u00eda  R\u00edo Negro Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Jos\u00e9","family":"Ponce","sequence":"additional","affiliation":[{"name":"Universidad Nacional de R\u00edo Negro (UNRN)  R\u00edo Negro Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  R\u00edo Negro Argentina"},{"name":"Servicio Geol\u00f3gico Minero Argentino (SEGEMAR), Centro General Roca  R\u00edo Negro Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mart\u00edn Nazareno","family":"Parada","sequence":"additional","affiliation":[{"name":"Universidad Nacional de R\u00edo Negro, Instituto de Investigaci\u00f3n en Paleobiolog\u00eda y Geolog\u00eda  R\u00edo Negro Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Investigaci\u00f3n en Paleobiolog\u00eda y Geolog\u00eda  R\u00edo Negro Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1144\/0016-76492010-025"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/684831"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-07886-x"},{"key":"e_1_2_9_5_1","unstructured":"Arregui C. 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