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Specialized anaerobic bacteria metabolize DCM; however, the genetic basis for this process has remained elusive. Comparative genomics of the three known anaerobic DCM\u2010degrading bacterial species revealed a homologous gene cluster, designated the methylene chloride catabolism (\n                    <jats:italic>mec<\/jats:italic>\n                    ) gene cassette, comprising 8\u201310\u00a0genes encoding proteins with 79.6%\u201399.7% amino acid identities. Functional annotation identified genes encoding a corrinoid\u2010dependent methyltransferase system, and shotgun proteomics applied to two DCM\u2010catabolizing cultures revealed high expression of proteins encoded on the\n                    <jats:italic>mec<\/jats:italic>\n                    gene cluster during anaerobic growth with DCM. In a DCM\u2010contaminated groundwater plume, the abundance of\n                    <jats:italic>mec<\/jats:italic>\n                    genes strongly correlated with DCM concentrations (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0=\u00a00.71\u20130.85) indicating their potential value as process\u2010specific bioremediation biomarkers.\n                    <jats:italic>mec<\/jats:italic>\n                    gene clusters were identified in metagenomes representing peat bogs, the deep subsurface, and marine ecosystems including oxygen minimum zones (OMZs), suggesting a capacity for DCM degradation in diverse habitats. The broad distribution of anaerobic DCM catabolic potential infers a role for DCM as an energy source in various environmental systems, and implies that the global DCM flux (i.e., the rate of formation minus the rate of consumption) might be greater than emission measurements suggest.\n                  <\/jats:p>","DOI":"10.1111\/gcb.16068","type":"journal-article","created":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T04:53:08Z","timestamp":1640839988000},"page":"2396-2412","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Identification and widespread environmental distribution of a gene cassette implicated in anaerobic dichloromethane degradation"],"prefix":"10.1111","volume":"28","author":[{"given":"Robert W.","family":"Murdoch","sequence":"first","affiliation":[{"name":"Center for Environmental Biotechnology University of Tennessee  Knoxville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gao","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology University of Tennessee  Knoxville Tennessee USA"},{"name":"Department of Civil and Environmental Engineering University of Tennessee  Knoxville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadime","family":"Kara Murdoch","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology University of Tennessee  Knoxville Tennessee USA"},{"name":"Biosciences Division Oak Ridge National Laboratory  Oak Ridge Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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