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While much research has focused on mean temperature increases, climate change also involves increasing variability of both water temperature and flow regimes and increasing concentrations of atmospheric CO<jats:sub>2<\/jats:sub>, all with potential to alter stream invertebrate communities. Deposited fine sediment is a pervasive land\u2010use stressor with widespread impacts on stream invertebrates. Sedimentation may be managed at the catchment scale; thus, uncovering interactions with these three key climate stressors may assist mitigation of future threats. This is the first experiment to investigate the individual and combined effects of enriched CO<jats:sub>2<\/jats:sub>, heatwaves, flow velocity variability, and fine sediment on realistic stream invertebrate communities. Using 128 mesocosms simulating small stony\u2010bottomed streams in a 7\u2010week experiment, we manipulated dissolved CO<jats:sub>2<\/jats:sub> (ambient; enriched), fine sediment (no sediment; 300\u2009g dry sediment), temperature (ambient; two 7\u2010day heatwaves), and flow velocity (constant; variable). All treatments changed community composition. CO<jats:sub>2<\/jats:sub> enrichment reduced abundances of Orthocladiinae and Chironominae and increased Copepoda abundance. Variable flow velocity had only positive effects on invertebrate abundances (7 of 13 common taxa and total abundance), in contrast to previous experiments showing negative impacts of reduced velocity. CO<jats:sub>2<\/jats:sub> was implicated in most stressor interactions found, with CO<jats:sub>2<\/jats:sub>\u2009\u00d7\u2009sediment interactions being most common. Communities forming under enriched CO<jats:sub>2<\/jats:sub> conditions in sediment\u2010impacted mesocosms had ~20% fewer total invertebrates than those with either treatment alone. Copepoda abundances doubled in CO<jats:sub>2<\/jats:sub>\u2010enriched mesocosms without sediment, whereas no CO<jats:sub>2<\/jats:sub> effect occurred in mesocosms with sediment. Our findings provide new insights into potential future impacts of climate change and land use in running freshwaters, in particular highlighting the potential for elevated CO<jats:sub>2<\/jats:sub> to interact with fine sediment deposition in unpredictable ways.<\/jats:p>","DOI":"10.1111\/gcb.17336","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T14:29:50Z","timestamp":1716388190000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Individual and combined impacts of carbon dioxide enrichment, heatwaves, flow velocity variability, and fine sediment deposition on stream invertebrate communities"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4525-1017","authenticated-orcid":false,"given":"Julia G.","family":"Hunn","sequence":"first","affiliation":[{"name":"Department of Zoology University of Otago  Dunedin New 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