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Time\u2010kill assay assessed growth kinetics of <jats:italic>C.\u00a0albicans<\/jats:italic> based on pre\u2010determined time points (0, 1, 2, 4, 6 and 24\u00a0hours). A multi\u2010species biofilm was formed using human saliva from patients with oral candidiasis and anti\u2010biofilm activity determined by Colony Forming Units per milliliter (CFU\/mL) count, fluorescence microscopy with calcofluor white to observe yeast presence and structure, and metabolic activity by XTT (2,3\u2010Bis\u2010(2Methoxy\u20104\u2010Nitro\u20105\u2010Sulfophenyl)\u20102H\u2010Tetrazolium\u20105\u2010carboxanilide) reduction assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The essential oil showed an anti\u2010fungal activity against all\u00a0<jats:italic>Candida<\/jats:italic>\u00a0species (MIC 500\u20101000\u00a0\u00b5g\/mL, MFC 1000\u20102000\u00a0\u00b5g\/mL), and the time\u2010kill assay showed that 2000\u00a0\u00b5g\/mL (from 2\u00a0hours onward) and 1000\u00a0\u00b5g\/mL (from 4\u00a0hours onward) concentrations had substantially lower yeast growth than the negative control. In the biofilm analysis, the essential oil had a lower CFU\/mL count and a biofilm metabolic activity (91.4%) than seen with its negative control, and in both analyses, the essential oil was not significantly different from the positive control (chlorhexidine). Morphological analysis showed amorphous and fragmented cellular structures after treatment with the essential oil.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>Syzygium aromaticum<\/jats:italic> essential oil had anti\u2010fungal activities, reduced the <jats:italic>Candida<\/jats:italic> growth kinetics substantially and inhibited the multi\u2010species biofilm formation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ger.12594","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T10:25:29Z","timestamp":1634207129000},"page":"366-373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["In vitro inhibition of a multispecies oral cavity biofilm by <i>Syzygium aromaticum<\/i> essential 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