{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T12:51:01Z","timestamp":1782305461146,"version":"3.54.5"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K18413"],"award-info":[{"award-number":["22K18413"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K19750"],"award-info":[{"award-number":["22K19750"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K21598"],"award-info":[{"award-number":["23K21598"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K27973"],"award-info":[{"award-number":["23K27973"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJSP2110"],"award-info":[{"award-number":["JPMJSP2110"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008732","name":"Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016926","name":"Kyoto University Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016926","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Geriatrics Gerontology Int"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Sarcopenia has recently become a major public health issue. Adrenomedullin (AM) exerts diverse physiological effects, including angiogenesis, but its role in skeletal muscle is unclear. This study investigates whether AM can attenuate sarcopenic changes in aged mice compared to young adults.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Young and old male C57BL\/6J mice were randomly divided into two groups: the AM\u2010treated group, which received subcutaneous administration of AM (50\u2009nmol\/kg body weight) 6\u2009days a week, and the sham\u2010treated group, which was injected with vehicle. After 3\u2009months of treatment, we performed multi\u2010muscle analyses (EDL, SOL, and PLA) using western blotting and immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>AM treatment significantly increased AM levels in plasma and muscle tissue. In young mice, AM was associated with changes in muscle fiber\u2010type composition, including a reduction in Type IIx fibers, suggesting muscle\u2010specific functional adaptation. In aged mice, AM increased CD31\u2010positive capillary density and Pax7\u2010positive muscle satellite cells (MuSCs), indicating improved vascular and stem cell niches. These changes occurred without alterations in muscle mass but were accompanied by improved motor function in aged mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>AM exerts age\u2010dependent effects on skeletal muscle, with distinct responses observed in young and aged mice. These findings suggest that AM may contribute to muscle adaptation through context\u2010dependent mechanisms involving vascular remodeling and fiber\u2010type modulation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ggi.70584","type":"journal-article","created":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T23:48:58Z","timestamp":1781480938000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Age\u2010Dependent Effects of Adrenomedullin on Muscle Fiber Composition, Angiogenesis, and Muscle Satellite Cells Maintenance"],"prefix":"10.1111","volume":"26","author":[{"given":"Ryota","family":"Iyama","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Adaptations to Exercise, Graduate School of Human and Environmental Studies Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eriko","family":"Kurogi","sequence":"additional","affiliation":[{"name":"Laboratory of Sports and Exercise Medicine, Graduate School of Human and Environmental Studies Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takumi","family":"Yokokawa","sequence":"additional","affiliation":[{"name":"Division of Food Science and Biotechnology, Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Department of Projects Research, Frontier Science Research Center University of Miyazaki  Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Sports and Exercise Medicine, Graduate School of Human and Environmental Studies Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9363-1589","authenticated-orcid":false,"given":"Tatsuro","family":"Egawa","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Adaptations to Exercise, Graduate School of Human and Environmental Studies Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)31138-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2014.01.005"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20240201"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000450922"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00461.2024"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00827.2005"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1249\/JES.0000000000000151"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2022.2090541"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2018.03.012"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology11071074"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(99)00128-X"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031877"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2021.101528"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12600"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803174105"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000153352.29335.B9"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddr265"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2008.03.003"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12221"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1249\/JES.0000000000000152"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.167197"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.113357"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01105.2004"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11663"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc4101.097111"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/44.3.571"}],"container-title":["Geriatrics &amp; Gerontology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ggi.70584","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ggi.70584","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ggi.70584","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T12:07:45Z","timestamp":1782302865000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ggi.70584"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":26,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/ggi.70584"],"URL":"https:\/\/doi.org\/10.1111\/ggi.70584","archive":["Portico"],"relation":{},"ISSN":["1444-1586","1447-0594"],"issn-type":[{"value":"1444-1586","type":"print"},{"value":"1447-0594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2025-05-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70584"}}