{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:33:33Z","timestamp":1788888813943,"version":"build-2803163510"},"reference-count":56,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,25]],"date-time":"2013-01-25T00:00:00Z","timestamp":1359072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:p>Synaptic transmission\u2010dependent regulation of neurotransmitter receptor accumulation at postsynaptic sites underlies the formation, maintenance and maturation of synaptic function. Previous <jats:italic>in vitro<\/jats:italic> studies showed that glycine receptor (GlyR) clustering requires synaptic inputs. However, <jats:italic>in vivo<\/jats:italic> GlyR regulation by synaptic transmission is not fully understood. Here, we established a model system using developing zebrafish, in which GlyRs are expressed in Mauthner cells (M\u2010cells), a pair of giant, reticulospinal, hindbrain neurons, thereby enabling analysis of GlyR clusters over time in identifiable cells. Bath application of a glycinergic blocker, strychnine, to developing zebrafish prevented postsynaptic GlyR cluster formation in the M\u2010cells. After strychnine removal, the GlyR clusters appeared in the M\u2010cells. At a later stage, glycinergic transmission blockade impaired maintenance of GlyR clusters. We also found that pharmacological blockade of either L\u2010type Ca<jats:sup>2+<\/jats:sup> channels or calcium\u2010\/calmodulin\u2010dependent protein kinase <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> (Ca<jats:styled-content style=\"fixed-case\">MKII<\/jats:styled-content>) disturbed GlyR clustering. In addition, the M\u2010cell\u2010specific Ca<jats:styled-content style=\"fixed-case\">MKII<\/jats:styled-content> inactivation using the Gal4\u2010<jats:styled-content style=\"fixed-case\">UAS<\/jats:styled-content> system significantly impaired GlyR clustering in the M\u2010cells. Thus, the formation and maintenance of GlyR clusters in the M\u2010cells in the developing animals are regulated in a synaptic transmission\u2010dependent manner, and Ca<jats:styled-content style=\"fixed-case\">MKII<\/jats:styled-content> activation at the postsynapse is essential for GlyR clustering. This is the first demonstration of synaptic transmission\u2010dependent modulation of synaptic GlyRs <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/gtc.12032","type":"journal-article","created":{"date-parts":[[2013,1,25]],"date-time":"2013-01-25T07:28:40Z","timestamp":1359098920000},"page":"211-224","source":"Crossref","is-referenced-by-count":39,"title":["Glycinergic transmission and postsynaptic activation of Ca<scp>MKII<\/scp> are required for glycine receptor clustering <i>in vivo<\/i>"],"prefix":"10.1111","volume":"18","author":[{"given":"Iori","family":"Yamanaka","sequence":"first","affiliation":[{"name":"Division of Biological Science Graduate School of Science Nagoya University Nagoya 464\u20108602 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariko","family":"Miki","sequence":"additional","affiliation":[{"name":"Division of Biological Science Graduate School of Science Nagoya University Nagoya 464\u20108602 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhide","family":"Asakawa","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology National Institute of Genetics Mishima 411\u20108540 Japan"},{"name":"Department of Genetics Graduate University for Advanced Studies Mishima 411\u20108540 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology National Institute of Genetics Mishima 411\u20108540 Japan"},{"name":"Department of Genetics Graduate University for Advanced Studies Mishima 411\u20108540 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoichi","family":"Oda","sequence":"additional","affiliation":[{"name":"Division of Biological Science Graduate School of Science Nagoya University Nagoya 464\u20108602 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Hirata","sequence":"additional","affiliation":[{"name":"Division of Biological Science Graduate School of Science Nagoya University Nagoya 464\u20108602 Japan"},{"name":"Department of Genetics Graduate University for Advanced Studies Mishima 411\u20108540 Japan"},{"name":"Center for Frontier Research National Institute of Genetics Mishima 411\u20108540 Japan"},{"name":"Precursory Research for Embryonic Science and Technology Japan Science and Technology Agency Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2000.84.4.1726"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704963105"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.04.023"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn920"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2645"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1077"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/stke.3272006pe13"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80555-3"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1088525"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2000.00271.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.04.010"},{"key":"e_1_2_6_13_1","volume-title":"Neurobiology of the Mauthner Cell","author":"Faber D. 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