{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T14:32:29Z","timestamp":1723041149565},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T00:00:00Z","timestamp":1374710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p><jats:styled-content style=\"fixed-case\">GATA<\/jats:styled-content>1 is a transcription factor essential for erythropoiesis and megakaryopoiesis. It has been found that <jats:styled-content style=\"fixed-case\"><jats:italic>Gata1<\/jats:italic><\/jats:styled-content> gene knockdown heterozygous female (<jats:styled-content style=\"fixed-case\"><jats:italic>Gata1<\/jats:italic><\/jats:styled-content><jats:sup><jats:italic>G1.05\/+<\/jats:italic><\/jats:sup>) mice spontaneously develop erythroblastic leukemias. In this study, we have generated a novel <jats:styled-content style=\"fixed-case\"><jats:italic>Gata1<\/jats:italic><\/jats:styled-content> knockdown erythroblastic cell line, designated <jats:styled-content style=\"fixed-case\">GAK<\/jats:styled-content>14, from the leukemia cells in the <jats:styled-content style=\"fixed-case\"><jats:italic>Gata1<\/jats:italic><\/jats:styled-content><jats:sup><jats:italic>G1.05\/+<\/jats:italic><\/jats:sup> mice. Although GAK14 cells maintain immature phenotype on <jats:styled-content style=\"fixed-case\">OP<\/jats:styled-content>9 stromal cells in the presence of erythropoietin and stem cell factor, the cells produce <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>r\u20101\u2010, <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>ac1\u2010, <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>220\u2010, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3e\u2010 or <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>49b\u2010positive hematopoietic cells when co\u2010cultured with <jats:styled-content style=\"fixed-case\">DAS<\/jats:styled-content>104\u20108 feeder cells. However, <jats:styled-content style=\"fixed-case\">GAK<\/jats:styled-content>14 cells did not produce erythroid and megakaryocytic lineages, perhaps due to the absence of <jats:styled-content style=\"fixed-case\">GATA<\/jats:styled-content>1. Indeed, <jats:styled-content style=\"fixed-case\">GAK<\/jats:styled-content>14 cells became capable of differentiating into mature erythroid cells when complemented with full\u2010length <jats:styled-content style=\"fixed-case\">GATA<\/jats:styled-content>1 and co\u2010cultured with fetal liver\u2013derived <jats:styled-content style=\"fixed-case\">FLS<\/jats:styled-content>5 stromal cells. This differentiation potential was impaired when <jats:styled-content style=\"fixed-case\">GATA<\/jats:styled-content>1 lacking the <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010terminal domain was complemented. The <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010terminal domain is known to contribute to the pathogenesis of transient abnormal myelopoiesis and acute megakaryoblastic leukemia related to <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>own syndrome. These results thus showed that <jats:styled-content style=\"fixed-case\">GAK<\/jats:styled-content>14 cells will serve as a powerful tool for dissecting domain function of <jats:styled-content style=\"fixed-case\">GATA<\/jats:styled-content>1 and that the <jats:styled-content style=\"fixed-case\">GATA<\/jats:styled-content>1 <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010terminal domain is essential for the erythroid differentiation of <jats:styled-content style=\"fixed-case\">GAK<\/jats:styled-content>14 cells.<\/jats:p>","DOI":"10.1111\/gtc.12084","type":"journal-article","created":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T15:39:04Z","timestamp":1374766744000},"page":"886-898","source":"Crossref","is-referenced-by-count":4,"title":["Establishment of erythroleukemic <scp>GAK<\/scp>14 cells and characterization of <scp>GATA<\/scp>1 <scp>N<\/scp>\u2010terminal domain"],"prefix":"10.1111","volume":"18","author":[{"given":"Harumi Y.","family":"Mukai","sequence":"first","affiliation":[{"name":"Graduate School of Comprehensive Human Sciences Center for Tsukuba Advanced Research Alliance University of Tsukuba Tsukuba 305\u20108577 Japan"},{"name":"Department of Medical Biochemistry Tohoku University Graduate School of Medicine Sendai 980\u20108575 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikiko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry Tohoku University Graduate School of Medicine Sendai 980\u20108575 Japan"},{"name":"Department of Molecular Hematology Tohoku University Graduate School of Medicine Sendai 980\u20108575 Japan"},{"name":"Center for Radioisotope Sciences Tohoku University Graduate School of Medicine Sendai 980\u20108575 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masumi","family":"Nagano","sequence":"additional","affiliation":[{"name":"Graduate School of Comprehensive Human Sciences 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