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In addition, rapamycin has been proposed as a calorie restriction mimetic to extend the life span of various organisms. The fission yeast <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>chizosaccharomyces pombe<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>.\u00a0pombe<\/jats:italic>) serves as a valuable genetic model system to study the mechanism(s) of drug action as well as to determine genetic contexts associated with drug sensitivity or resistance. Here, we identified genes that when deleted modulate the rapamycin\u2010sensitive strains in <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>.\u00a0pombe<\/jats:italic>. We carried out a chemical genomics screen for rapamycin\u2010sensitive mutants using the genome\u2010deletion library which covers 95.3% of all nonessential fission yeast genes and confirmed 59 genes to be rapamycin sensitive. Gene Ontology (GO) enrichment analysis showed that strains sensitive to rapamycin are highly enriched in processes regulating t<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> modification and mitochondria as well as other ontologies, including cellular metabolic process, chromatin organization, cell cycle, signaling, translation, transport and other cellular processes. Analysis also showed that components of the Elongator complex are overrepresented in the sensitive strains. Here, the data obtained will provide valuable information for speculation on the actions of rapamycin as well as on TORC signaling, thereby presenting a strategy to enhance sensitivity to rapamycin.<\/jats:p>","DOI":"10.1111\/gtc.12223","type":"journal-article","created":{"date-parts":[[2015,2,5]],"date-time":"2015-02-05T08:03:39Z","timestamp":1423123419000},"page":"292-309","source":"Crossref","is-referenced-by-count":19,"title":["Chemical genomics approach to identify genes associated with sensitivity to rapamycin in the fission yeast <i>Schizosaccharomyces pombe<\/i>"],"prefix":"10.1111","volume":"20","author":[{"given":"Akira","family":"Doi","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Pharmacogenomics School of Pharmaceutical Sciences Kinki University Kowakae 3\u20104\u20101 Higashi\u2010Osaka 577\u20108502 Japan"},{"name":"Japan Society for the Promotion of Science 1\u20108 Chiyoda\u2010ku Tokyo 102\u20108472 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayumi","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacogenomics School of Pharmaceutical Sciences Kinki University Kowakae 3\u20104\u20101 Higashi\u2010Osaka 577\u20108502 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Sato","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacogenomics School of Pharmaceutical Sciences Kinki University Kowakae 3\u20104\u20101 Higashi\u2010Osaka 577\u20108502 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaya","family":"Uno","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacogenomics School of Pharmaceutical Sciences Kinki University Kowakae 3\u20104\u20101 Higashi\u2010Osaka 577\u20108502 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Kanda","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacogenomics School of Pharmaceutical Sciences Kinki University Kowakae 3\u20104\u20101 Higashi\u2010Osaka 577\u20108502 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Asami","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacogenomics School of Pharmaceutical Sciences Kinki University Kowakae 3\u20104\u20101 Higashi\u2010Osaka 577\u20108502 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuriko","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacogenomics School of Pharmaceutical Sciences Kinki University Kowakae 3\u20104\u20101 Higashi\u2010Osaka 577\u20108502 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayako","family":"Kita","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacogenomics School of Pharmaceutical Sciences Kinki University Kowakae 3\u20104\u20101 Higashi\u2010Osaka 577\u20108502 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Satoh","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacogenomics School of Pharmaceutical Sciences Kinki University Kowakae 3\u20104\u20101 Higashi\u2010Osaka 577\u20108502 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reiko","family":"Sugiura","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacogenomics School of Pharmaceutical Sciences Kinki University Kowakae 3\u20104\u20101 Higashi\u2010Osaka 577\u20108502 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E07-03-0274"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200767030-00004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.10.22.18329"},{"key":"e_1_2_6_5_1","first-page":"27","article-title":"Rapamycin and mTOR kinase inhibitors","volume":"1","author":"Ballou L.M.","year":"2008","journal-title":"J. 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