{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T15:06:02Z","timestamp":1790262362182,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,1,8]],"date-time":"2016-01-08T00:00:00Z","timestamp":1452211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19658040"],"award-info":[{"award-number":["19658040"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22658036"],"award-info":[{"award-number":["22658036"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2016,2]]},"abstract":"The T\u2010box transcription factors T\u2010bet and eomesodermin (Eomes) have been shown to regulate the lineage\u2010specific expression of interferon\u2010\u03b3 (IFN<\/jats:styled-content>\u2010\u03b3). However, in contrast to T\u2010bet, the role of Eomes in the expression of IFN<\/jats:styled-content>\u2010\u03b3 remains unclear. In this study, we investigated the Eomes\u2010dependent expression of IFN<\/jats:styled-content>\u2010\u03b3 in the mouse thymoma BW<\/jats:styled-content>5147 and EL<\/jats:styled-content>4 cells, which do not express T\u2010bet or Eomes. The ectopic expression of Eomes induced BW<\/jats:styled-content>5147 and EL<\/jats:styled-content>4 cells to produce IFN<\/jats:styled-content>\u2010\u03b3 in response to phorbol 12\u2010myristate 13\u2010acetate (PMA<\/jats:styled-content>) and ionomycin (IM<\/jats:styled-content>). In BW<\/jats:styled-content>5147 cells, Eomes augmented luciferase activity driven by the Ifng<\/jats:italic> promoter encoding from \u20132500 to +113\u00a0bp; however, it was not increased by a stimulation with PMA<\/jats:styled-content> and IM<\/jats:styled-content>. A chromatin immunoprecipitation assay showed that Eomes bound to the Ifng<\/jats:italic> promoter and conserved noncoding sequence (CNS<\/jats:styled-content>) \u201322\u00a0kb across the Ifng<\/jats:italic> locus with high efficacy in BW<\/jats:styled-content>5147 cells. Moreover, Eomes increased permissive histone modifications in the Ifng<\/jats:italic> promoter and multiple CNS<\/jats:styled-content>s. The stimulation with PMA<\/jats:styled-content> and IM<\/jats:styled-content> greatly augmented Eomes binding to CNS<\/jats:styled-content>\u201354, CNS<\/jats:styled-content>\u201334, CNS<\/jats:styled-content>+19 and CNS<\/jats:styled-content>+30, which was inhibited by FK<\/jats:styled-content>506. These results indicated that Eomes bound to the Ifng<\/jats:italic> promoter and multiple CNS<\/jats:styled-content>s in stimulation\u2010dependent and stimulation\u2010independent manners.<\/jats:p>","DOI":"10.1111\/gtc.12328","type":"journal-article","created":{"date-parts":[[2016,1,10]],"date-time":"2016-01-10T13:48:20Z","timestamp":1452433700000},"page":"146-162","source":"Crossref","is-referenced-by-count":17,"title":["Eomesodermin promotes interferon\u2010\u03b3 expression and binds to multiple conserved noncoding sequences across the Ifng<\/i> locus in mouse thymoma cell lines"],"prefix":"10.1111","volume":"21","author":[{"given":"Natsuki","family":"Fukuoka","sequence":"first","affiliation":[{"name":"Department of Applied Biology Kyoto Institute of Technology Matsugasaki Sakyo\u2010ku Kyoto 606\u20108585 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misuzu","family":"Harada","sequence":"additional","affiliation":[{"name":"Department of Applied Biology 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