{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T18:48:34Z","timestamp":1782326914674,"version":"3.54.5"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T00:00:00Z","timestamp":1518566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p>Periodontal disease, an inflammatory disease, is caused by infection with periodontal pathogens. Long\u2010term periodontal disease increases the risk of oral carcinogenesis. Similar to other peptic cancers, oral carcinogenesis also requires multiple genome instabilities; however, the risk factors related to the accumulation of genome instabilities are poorly understood. Here, we suggested that specific periodontal pathogens may increase the risk of genome instability. Accordingly, we screened several periodontal pathogens based on the ability to induce DNA double\u2010strand breaks (DSBs) in host cells. We found that <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic> Y4 infection induced DSB formation in host cells. To assess whether DSB formation induced by infection with <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic> occurred through apoptotic chromosome fragmentation, cells were treated with a caspase inhibitor, Z\u2010VAD\u2010FMK. DSB accumulation induced by infection with <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic> was observed, even in the presence of Z\u2010VAD\u2010FMK, suggesting that this breakage occurred independently of apoptosis. These results suggested that some periodontal pathogens can increase the risk of genome instabilities in host cells and subsequently increase the risk of carcinogenesis.<\/jats:p>","DOI":"10.1111\/gtc.12570","type":"journal-article","created":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T06:26:40Z","timestamp":1518589600000},"page":"264-273","source":"Crossref","is-referenced-by-count":17,"title":["<i>Aggregatibacter actinomycetemcomitans<\/i> infection causes DNA double\u2010strand breaks in host cells"],"prefix":"10.1111","volume":"23","author":[{"given":"Rie","family":"Teshima","sequence":"first","affiliation":[{"name":"Department of Environmental and Preventive Medicine Faculty of Medicine Oita University  Oita Japan"},{"name":"Department of Oral and Maxillo\u2010Facial Surgery Faculty of Medicine Oita University  Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiro","family":"Hanada","sequence":"additional","affiliation":[{"name":"Department of Environmental and Preventive Medicine Faculty of Medicine Oita University  Oita Japan"},{"name":"Clinical Engineering Research Center Faculty of Medicine Oita University  Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junko","family":"Akada","sequence":"additional","affiliation":[{"name":"Department of Environmental and Preventive Medicine Faculty of Medicine Oita University  Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Kawano","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillo\u2010Facial Surgery Faculty of Medicine Oita University  Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1222-5819","authenticated-orcid":false,"given":"Yoshio","family":"Yamaoka","sequence":"additional","affiliation":[{"name":"Department of Environmental and Preventive Medicine Faculty of Medicine Oita University  Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells3020592"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.05.010"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2013.05.001"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1128\/jcm.32.1.75-81.1994","article-title":"Identification and characterization of genetic cluster groups of Actinobacillus actinomycetemcomitans isolated from the human oral cavity","volume":"32","author":"DiRienzo J. 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