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CEP161 is presumably a phosphoprotein and a component of the Hippo pathway. The interaction site was located in the N\u2010terminal half of CEP161 which encompasses the \u03b3TURC binding domain in CEP161. This binding domain is responsible for binding of the \u03b3\u2010tubulin ring complex which allows microtubule nucleation at the centrosome. GFP\u2010tagged phr2AB is diffusely distributed throughout the cell and enriched at the centrosome. Ectopic expression of phr2AB as GFP fusion protein led to multinucleation, aberrant nucleus centrosome ratios and an altered sensitivity to okadaic acid. Some of these features were also affected in cells over\u2010expressing domains of CEP161 and in cells from patients suffering from primary microcephaly, which carried a mutated <jats:italic>CDK5RAP2<\/jats:italic> gene.<\/jats:p>","DOI":"10.1111\/gtc.12637","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T19:49:27Z","timestamp":1534967367000},"page":"923-931","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The regulatory subunit phr2AB of <i>Dictyostelium discoideum<\/i> phosphatase PP2A interacts with the centrosomal protein CEP161, a CDK5RAP2 ortholog"],"prefix":"10.1111","volume":"23","author":[{"given":"Rolf","family":"M\u00fcller","sequence":"first","affiliation":[{"name":"Institute for Biochemistry I, Medical Faculty University Hospital Cologne Cologne Germany"},{"name":"Center for Molecular Medicine Cologne and Cologne Cluster on Cellular Stress Responses in Aging\u2010Associated Diseases, Medical Faculty University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Stumpf","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I, Medical Faculty University Hospital Cologne Cologne Germany"},{"name":"Center for Molecular Medicine Cologne and Cologne Cluster on Cellular Stress Responses in Aging\u2010Associated Diseases, Medical Faculty University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina","family":"Wehrstedt","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I, Medical Faculty University Hospital Cologne Cologne Germany"},{"name":"Center for Molecular Medicine Cologne and Cologne Cluster on Cellular Stress Responses in Aging\u2010Associated Diseases, Medical Faculty University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salil K.","family":"Sukumaran","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I, Medical Faculty University Hospital Cologne Cologne Germany"},{"name":"Center for Molecular Medicine Cologne and Cologne Cluster on Cellular Stress Responses in Aging\u2010Associated Diseases, Medical Faculty University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malte A.","family":"Karow","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I, Medical Faculty University Hospital Cologne Cologne Germany"},{"name":"Center for Molecular Medicine Cologne and Cologne Cluster on Cellular Stress Responses in Aging\u2010Associated Diseases, Medical Faculty University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marija","family":"Marko","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I, Medical Faculty University Hospital Cologne Cologne Germany"},{"name":"Center for Molecular Medicine Cologne and Cologne Cluster on Cellular Stress Responses in Aging\u2010Associated Diseases, Medical Faculty University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8260-6732","authenticated-orcid":false,"given":"Angelika A.","family":"Noegel","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I, Medical Faculty University Hospital Cologne Cologne Germany"},{"name":"Center for Molecular Medicine Cologne and Cologne Cluster on Cellular Stress Responses in Aging\u2010Associated Diseases, Medical Faculty University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludwig","family":"Eichinger","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I, Medical Faculty University Hospital Cologne Cologne Germany"},{"name":"Center for Molecular Medicine Cologne and Cologne Cluster on Cellular Stress Responses in Aging\u2010Associated Diseases, Medical Faculty University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20021964"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.087106"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-03-0180"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201606033"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3576222"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00208-14"},{"key":"e_1_2_6_8_1","unstructured":"Dinno A.(2017)dunn.test: Dunn's test of multiple comparisons using rank sums. 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