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Immunoblotting has displayed weak FEAT expression in normal human tissues, including the testis. Here, we found that FEAT is expressed in fetal and adult Leydig cells in the testis. FEAT knockdown using siRNA increased primary cilia formation in MA\u201010 Leydig tumor cells, accompanied by enhanced 5\u2032 adenosine monophosphate\u2010activated protein kinase (AMPK) activation. Immunofluorescence analyses of FEAT\u2010silenced MA\u201010 cells showed diminished insulin\u2010like factor 3 (INSL3) expression. A male<jats:italic>Mettl13<jats:sup>+\/\u2212<\/jats:sup><\/jats:italic>mouse developed bilateral intraabdominal cryptorchidism, suggesting defective INSL3 production by fetal Leydig cells. Leydig cells from the mouse showed markedly decreased INSL3 protein by immunohistochemistry. Together, these results suggest that FEAT facilitates the INSL3 production in testicular Leydig cells that is essential for transabdominal testis migration.<\/jats:p>","DOI":"10.1111\/gtc.12644","type":"journal-article","created":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T07:08:15Z","timestamp":1536044895000},"page":"952-962","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["FEAT enhances INSL3 expression in testicular Leydig cells"],"prefix":"10.1111","volume":"23","author":[{"given":"Yan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Hematology and Oncology, Graduate School of Medicine Kyoto University Kyoto Japan"},{"name":"Division of Molecular and Clinical Genetics Medical Institute of Bioregulation, Kyushu University Fukuoka Japan"},{"name":"Research Institute of Health and Welfare Kibi International University Takahashi Okayama 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