{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T02:00:52Z","timestamp":1788314452019,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,4,14]],"date-time":"2020-04-14T00:00:00Z","timestamp":1586822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"JSPS KAKENHI","award":["7H05760"],"award-info":[{"award-number":["7H05760"]}]},{"name":"JSPS KAKENHI","award":["18K16949"],"award-info":[{"award-number":["18K16949"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Di\u2010 and trimethylation of lysine 27 on histone 3 (H3K27me2\/3) is a critical gene repression mechanism. We previously showed that down\u2010regulation of the H3K27 demethylase, Jumonji domain\u2010containing protein 3 (JMJD3), resulted in a reduced number of protein kinase C (PKC)\u03b1\u2010positive rod ON\u2010bipolar cells. In this work, we focused on the role of another H3K27 demethylase, ubiquitously transcribed tetratricopeptide repeat X chromosome (UTX), in retinal development. UTX was expressed in the retinal progenitor cells of the embryonic mouse retina and was observed in the inner nuclear layer during late retinal development and in the mature retina. The short hairpin RNA\u2010mediated knockdown of <jats:italic>Utx<\/jats:italic> in a mouse retinal explant led to a reduced number of PKC\u03b1\u2010positive rod ON\u2010bipolar cells. However, other retinal subtypes were unaffected by this knockdown. Using a retina\u2010specific knockout of <jats:italic>Utx<\/jats:italic> in mice, the in vivo effects of UTX down\u2010regulation were examined. Again, the number of PKC\u03b1\u2010positive rod ON\u2010bipolar cells was reduced, and no other apparent phenotypes, including retinal progenitor proliferation, apoptosis or differentiation, were observed. Finally, we examined retina\u2010specific <jats:italic>Utx<\/jats:italic> and <jats:italic>Jmjd3<\/jats:italic> double\u2010knockout mice and found that although the number of rod ON\u2010bipolar cells was reduced, no additional effects from the loss of <jats:italic>Utx<\/jats:italic> and <jats:italic>Jmjd3<\/jats:italic> were observed. Taken together, our data show that UTX contributes to retinal differentiation in a lineage\u2010specific manner.<\/jats:p>","DOI":"10.1111\/gtc.12767","type":"journal-article","created":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T02:55:39Z","timestamp":1585191339000},"page":"402-412","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["H3K27me3 demethylase UTX regulates the differentiation of a subset of bipolar cells in the mouse retina"],"prefix":"10.1111","volume":"25","author":[{"given":"Daisy","family":"Umutoni","sequence":"first","affiliation":[{"name":"Division of Molecular and Developmental Biology Institute of Medical Science University of Tokyo Tokyo Japan"},{"name":"Department of Ophthalmology University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiro","family":"Iwagawa","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology Institute of Medical Science University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiro","family":"Baba","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology Institute of Medical Science University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asano","family":"Tsuhako","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology Institute of Medical Science University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Honda","sequence":"additional","affiliation":[{"name":"Field of Human Disease Models Major in Advanced Life Sciences and Medicine Institute of Laboratory Animals Tokyo Women\u2019s Medical University Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Aihara","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4497-5750","authenticated-orcid":false,"given":"Sumiko","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology Institute of Medical Science University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.088096"},{"key":"e_1_2_6_3_1","first-page":"381","article-title":"Regulation of chromatin by histone modifications","volume":"21","author":"Bannister A. 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