{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:25:54Z","timestamp":1759742754961,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T00:00:00Z","timestamp":1589155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Yongchuan District 2017 Joint Funding Project","award":["Ycstc\uff0c2017nc5022"],"award-info":[{"award-number":["Ycstc\uff0c2017nc5022"]}]},{"name":"National Major New Drug Innovation and Production Project","award":["2011ZX09401-308-26"],"award-info":[{"award-number":["2011ZX09401-308-26"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nasopharyngeal carcinoma (NPC) is a malignant tumor in nasopharynx tissues and lacks effective treatment strategies. Dysregulation of distal\u2010less homeobox 4 (DLX4) participates in the development of tumors. Understanding the regulatory mechanism of DLX4 in NPC progression may address this issue. Here, we first identified an up\u2010regulation of DLX4 in NPC cell lines compared to normal epithelial cells. Data from colony formation and transwell assays showed that knockdown of DLX4 inhibited cell proliferation and invasion of NPC, respectively. Moreover, DLX4 knockdown blocked the cell cycle of NPC at G1 phase, suggesting the antitumor effect of DLX4 knockdown on NPC. The downstream target of DLX4 was identified as Y\u2010box binding protein 1 (YB\u20101), whose expression was increased by over\u2010expression of DLX4, while decreased by knockdown of DLX4. The binding capacity between DLX4 and YB\u20101 was verified by chromatin immunoprecipitation (ChIP), and the result showed that DLX4 could not directly bind to the promoter of YB\u20101. Mechanically, YB\u20101 over\u2010expression reversed the effects of DLX4 knockdown on cell proliferation, cell cycle arrest and cell invasion of NPC. In conclusion, our findings indicated that DLX4 promoted NPC progression via up\u2010regulation of YB\u20101, which would shed light on therapeutic schedule in NPC.<\/jats:p>","DOI":"10.1111\/gtc.12772","type":"journal-article","created":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T04:14:57Z","timestamp":1586751297000},"page":"466-474","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Homeodomain protein DLX4 facilitates nasopharyngeal carcinoma progression via up\u2010regulation of YB\u20101"],"prefix":"10.1111","volume":"25","author":[{"given":"Zeyi","family":"Ling","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Head and neck surgery Yongchuan Hospital of Chongqing Medical University Chongqing City China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3305-4027","authenticated-orcid":false,"given":"Xiaoli","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Geriatrics Yongchuan Hospital of Chongqing Medical University Chongqing City China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and neck surgery Yongchuan Hospital of Chongqing Medical University Chongqing City China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingliang","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and neck surgery Yongchuan Hospital of Chongqing Medical University Chongqing City China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,11]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_12_2_1","DOI":"10.1016\/j.ejca.2009.12.024"},{"doi-asserted-by":"publisher","key":"e_1_2_12_3_1","DOI":"10.3322\/caac.21492"},{"doi-asserted-by":"publisher","key":"e_1_2_12_4_1","DOI":"10.5732\/cjc.010.10377"},{"doi-asserted-by":"publisher","key":"e_1_2_12_5_1","DOI":"10.1186\/1471-2407-11-370"},{"doi-asserted-by":"publisher","key":"e_1_2_12_6_1","DOI":"10.1128\/MCB.22.8.2505-2514.2002"},{"doi-asserted-by":"publisher","key":"e_1_2_12_7_1","DOI":"10.4161\/cc.5.11.2784"},{"doi-asserted-by":"publisher","key":"e_1_2_12_8_1","DOI":"10.4149\/neo_2014_041"},{"doi-asserted-by":"publisher","key":"e_1_2_12_9_1","DOI":"10.1002\/ame2.12002"},{"doi-asserted-by":"publisher","key":"e_1_2_12_10_1","DOI":"10.1126\/science.1151651"},{"issue":"3","key":"e_1_2_12_11_1","first-page":"607","article-title":"Y\u2010box binding 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