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The current diagnosis of Chikungunya virus (CHIKV) relies on a conventional reverse transcription polymerase chain reaction assay. Reverse transcription loop\u2010mediated isothermal amplification (RT\u2010LAMP) is a rapid and simple tool used for DNA\u2010based diagnosis of a variety of infectious diseases. In this study, we established an RT\u2010LAMP system to recognize CHIKV by targeting the envelope protein 1 (E1) gene that could also detect CHIKV at a concentration of 8 PFU without incorrectly detecting other mosquito\u2010borne viruses. The system also amplified the E1 genome in the serum of CHIKV\u2010infected mice with high sensitivity and specificity. Moreover, we established a dry RT\u2010LAMP system that can be transported without a cold chain, which detected the virus genome in CHIKV\u2010infected patient samples with high accuracy. 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