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In <jats:italic>Drosophila<\/jats:italic>, the level of ecdysone titer oscillates and developmental stage transitions, such as larval molting and metamorphosis, are induced at each of ecdysone peaks. Ecdysone titer also peaks at the stage of mid\u2010embryogenesis and the embryonic ecdysone is necessary for morphogenesis of several organs, although the regulatory mechanisms of embryonic organogenesis dependent on ecdysone signaling are still open questions. In this study, we find that absence or interruption of embryonic ecdysone signaling caused multiple defects in the tracheal system, including decrease in luminal protein deposition, uneven dilation of the dorsal trunk and loss of terminal branches. We also reveal that an ecdysone\u2010inducible gene <jats:italic>polished rice<\/jats:italic> (<jats:italic>pri<\/jats:italic>) is essential for tip cell fate decision in dorsal branches. As over\u2010expression of <jats:italic>pri<\/jats:italic> can restore the defects caused by disturbance of ecdysone biosynthesis, <jats:italic>pri<\/jats:italic> functions as one of the major mediators of embryonic ecdysone signal in tracheogenesis. These results demonstrate that ecdysone and its downstream target <jats:italic>pri<\/jats:italic> play essential roles in tracheal development by modulating cell fate decision.<\/jats:p>","DOI":"10.1111\/gtc.12841","type":"journal-article","created":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T17:38:51Z","timestamp":1614101931000},"page":"269-281","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<i>polished rice<\/i> mediates ecdysone\u2010dependent control of <i>Drosophila<\/i> embryonic organogenesis"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6810-3522","authenticated-orcid":false,"given":"Yuki","family":"Taira","sequence":"first","affiliation":[{"name":"Department of Biology Graduate School of Science Kobe University Kobe 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