{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:29:34Z","timestamp":1787243374429,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T00:00:00Z","timestamp":1635379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["GM131768"],"award-info":[{"award-number":["GM131768"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["NS114458"],"award-info":[{"award-number":["NS114458"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["GM123266"],"award-info":[{"award-number":["GM123266"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Many human diseases, including cancer and neurological abnormalities, are linked to deficiencies of phosphatase and tensin homolog deleted on chromosome ten (PTEN), a dual phosphatase that dephosphorylates both lipids and proteins. PTEN functions in multiple intracellular locations, including the plasma membrane and nucleus. Therefore, a critical challenge to understand the pathogenesis of PTEN\u2010associated diseases is to determine the specific role of PTEN at different locations. Toward this goal, the current study generated a mouse line in which lysine 13, which is critical for the nuclear localization of PTEN, is changed to arginine in the lipid\u2010binding domain using the CRISPR\u2010Ca9 gene\u2010editing system. We found that PTEN\n                    <jats:sub>K13R<\/jats:sub>\n                    mice show a strong decrease in the localization of PTEN in the nucleus without affecting the protein stability, phosphatase activity, and phosphorylation in the C\u2010terminal tail region. PTEN\n                    <jats:sub>K13R<\/jats:sub>\n                    mice are viable but produce smaller neurons and develop microcephaly. These data demonstrate that PTEN\n                    <jats:sub>K13R<\/jats:sub>\n                    mice provide a useful animal model to study the role of PTEN in the nucleus in vivo.\n                  <\/jats:p>","DOI":"10.1111\/gtc.12902","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T14:26:12Z","timestamp":1634739972000},"page":"1014-1022","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Generating a new mouse model for nuclear PTEN deficiency by a single K13R mutation"],"prefix":"10.1111","volume":"26","author":[{"given":"Takashi","family":"Kato","sequence":"first","affiliation":[{"name":"Department of Cell Biology Johns Hopkins University School of Medicine Baltimore MD USA"},{"name":"Faculty of Pharmacy Yasuda Women's University Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Igarashi","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Sesaki","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3073-9135","authenticated-orcid":false,"given":"Miho","family":"Iijima","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng782"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.12.023"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1236188"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1004(200008)16:2<109::AID-HUMU3>3.0.CO;2-0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddl177"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2015.339"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.239"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2014.10.015"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-6331-9_6"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402073"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509939103"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-13-2661"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.002356"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.03.081"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201488658"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.02.093"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101548"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.238"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2014.00023"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.03.023"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng781"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20150224"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5308.1943"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(03)00248-4"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.70.1.247"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.14.3513"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.507"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1409433111"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12600"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.293"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.022459"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.04.013"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.11.042"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.12.020"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3330"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81780-8"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.01.030"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.015230"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2016.06.025"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2014.10.018"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.493"}],"container-title":["Genes to Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/gtc.12902","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/gtc.12902","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/gtc.12902","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T12:25:26Z","timestamp":1693225526000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/gtc.12902"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,28]]},"references-count":41,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1111\/gtc.12902"],"URL":"https:\/\/doi.org\/10.1111\/gtc.12902","archive":["Portico"],"relation":{},"ISSN":["1356-9597","1365-2443"],"issn-type":[{"value":"1356-9597","type":"print"},{"value":"1365-2443","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,28]]},"assertion":[{"value":"2021-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}