{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T03:55:42Z","timestamp":1781754942455,"version":"3.54.5"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["KAKENHI Grant Number 19H04265"],"award-info":[{"award-number":["KAKENHI Grant Number 19H04265"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The structure\u2010specific endonuclease XPF\u2010ERCC1 is a multi\u2010functional heterodimer that participates in a variety of DNA repair mechanisms for maintaining genome integrity. Both subunits contain C\u2010terminal tandem helix\u2013hairpin\u2013helix (HhH<jats:sub>2<\/jats:sub>) domains, which are necessary for not only their dimerization but also enzymatic activity as well as protein stability. However, the interdependency of both subunits in their nuclear localization remains poorly understood. In this study, we have analyzed the region(s) that affects the subcellular localization of XPF and ERCC1 using various deletion mutants. We first identified the nuclear localization signal (NLS) in XPF, which was essential for its nuclear localization under the ERCC1\u2010free condition, but dispensable in the presence of ERCC1 (probably as XPF\u2010ERCC1 heterodimer). Interestingly, in the NLS\u2010independent and ERCC1\u2010dependent XPF nuclear localization, the physical interaction between XPF and ERCC1 via C\u2010terminal HhH<jats:sub>2<\/jats:sub> domains was not needed. Instead, the amino acid regions 311\u2013469 of XPF and 216\u2013260 of ERCC1 are required for the nuclear localization. Furthermore, we found that the 311\u2013469 region of XPF interacts with ERCC1 in a co\u2010immunoprecipitation assay. These results suggest that the nuclear localization of XPF\u2010ERCC1 heterodimer is regulated at multiple levels in an interdependent manner.<\/jats:p>","DOI":"10.1111\/gtc.12932","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T09:52:33Z","timestamp":1646301153000},"page":"356-367","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mapping of the regions implicated in nuclear localization of multi\u2010functional <scp>DNA<\/scp> repair endonuclease <scp>XPF\u2010ERCC1<\/scp>"],"prefix":"10.1111","volume":"27","author":[{"given":"Ryo","family":"Akahori","sequence":"first","affiliation":[{"name":"Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chie","family":"Takamori","sequence":"additional","affiliation":[{"name":"Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuo","family":"Wakasugi","sequence":"additional","affiliation":[{"name":"Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0828-0783","authenticated-orcid":false,"given":"Tsukasa","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences Kanazawa University  Kanazawa 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