{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:11:02Z","timestamp":1747185062340,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,21]],"date-time":"2022-08-21T00:00:00Z","timestamp":1661040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>TAP is a general mRNA export receptor and is highly conserved among eukaryotes. The nematode <jats:italic>Caenorhabditis elegans<\/jats:italic> has another TAP\u2010like protein, NXF\u20102, but little is known about its function. In this study, we show that NXF\u20102 is specifically expressed in germ cells and forms a novel granular structure that is different from that of P granules and that NXF\u20102 granules are anchored to the nuclear periphery in the mitotic region of the hermaphrodite gonad. In contrast, NXF\u20102 granules are released within the whole cytoplasm in the meiotic region, where the feminization gene <jats:italic>tra\u20102<\/jats:italic> starts to function. Both inhibition of XPO\u20101 (an ortholog of the export receptor CRM1) and mutation of the nuclear export signal of NXF\u20102 caused the release of NXF\u20102 granules from the nuclear periphery, indicating that anchoring of NXF\u20102 granules depends on XPO\u20101 function. Moreover, inhibition of NXF\u20102 resulted in a substantial nuclear accumulation of the reporter mRNA carrying the <jats:italic>tra\u20102<\/jats:italic> 3\u2032UTR. These results suggest that, together with XPO\u20101, NXF\u20102 exports and anchors <jats:italic>tra\u20102<\/jats:italic> mRNA to the nuclear periphery to avoid precocious translation until the germ cells reach the meiotic region, thereby contributing to the regulation of <jats:italic>tra\u20102<\/jats:italic> mRNA expression.<\/jats:p>","DOI":"10.1111\/gtc.12978","type":"journal-article","created":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T14:19:53Z","timestamp":1660227593000},"page":"621-628","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The germ cell\u2010specific <scp>TAP<\/scp>\u2010like protein <scp>NXF<\/scp>\u20102 forms a novel granular structure and is required for <i>tra\u20102<\/i> 3\u2032<scp>UTR<\/scp>\u2010dependent <scp>mRNA<\/scp> export in <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7004-4031","authenticated-orcid":false,"given":"Takashi","family":"Miwa","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science Kobe University Graduate School of Science  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keigo","family":"Ohtani","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Kobe University Graduate School of Science  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunio","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Kobe University Graduate School of Science  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Kobe University Graduate School of Science  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.147"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12962"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.24.5265"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1895\/wormbook.1.82.2"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.07.007"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.248"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80074-O"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201010106"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.23.8996-9008.2000"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/114.1.15"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.04.003"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.1.258"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00419-5"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes6020163"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.11.027"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2255"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.05.004"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.3.4.461"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzh062"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.853700"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.11.1453"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0030006"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.6.9.1159"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9607"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.044255"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.01298-12"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.5.1558"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90203-9"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1355838200000832"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/ede.12039"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.109.008292"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064144"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.047654"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00303-3"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008338"}],"container-title":["Genes to Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/gtc.12978","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/gtc.12978","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/gtc.12978","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T09:17:53Z","timestamp":1692695873000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/gtc.12978"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,21]]},"references-count":35,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1111\/gtc.12978"],"URL":"https:\/\/doi.org\/10.1111\/gtc.12978","archive":["Portico"],"relation":{},"ISSN":["1356-9597","1365-2443"],"issn-type":[{"type":"print","value":"1356-9597"},{"type":"electronic","value":"1365-2443"}],"subject":[],"published":{"date-parts":[[2022,8,21]]},"assertion":[{"value":"2022-07-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}